scpA, a new salicylate hydroxylase gene localized in salicylate/caprolactam degradation plasmids

• Published in: Molecular biology (New York) Vol. 47; no. 1; pp. 105 - 111
• Main Authors: Panov, A. V., Volkova, O. V., Puntus, I. F., Esikova, T. Z., Kosheleva, I. A., Boronin, A. M.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 2013; Springer Nature B.V
• Subjects: Bacteria; Biochemistry; Biodegradation; Biomedical and Life Sciences; Chemical contaminants; Genomics; Genomics. Transcriptomics; Human Genetics; Life Sciences; Molecular biology; Plasmids; Pseudomonas; plasmid; salicylate 1-hydroxylase; caprolactam; gene; and
• ISSN: 0026-8933; 1608-3245
• DOI: 10.1134/S0026893313010147

Both caprolactams and salicylate biodegradation by
Pseudomonas
salicylate/caprolactam degraders are controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids have been assigned to the P-7 incompatibility group. The new salicylate 1-hydroxylase gene (
scpA
) has been detected in SAL/CAP plasmids and partially sequenced. The
scpA
gene was equally related to the closest homolog genes
nahG
(NAH7),
salA
(
P. reinekei
MT1), and
nahU
(pND6-1); however, the identity rate did not exceed 72–74%. The synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited the highest specific activity toward 4-methylsalicylate and nonsubstituted salicylate substrates. Furthermore, conjugative pseudomonads’ plasmids of salicylate degradation without the classical
nah2
operon, which harbors only salicylate 1-hydroxylase gene
nahU
have been described for the first time.