Effect of adding a mycotoxin-sequestering agent on milk aflatoxin M₁ concentration and the performance and immune response of dairy cattle fed an aflatoxin B₁-contaminated diet

• Published in: Journal of dairy science Vol. 95; no. 10; p. 5901
• Main Authors: Queiroz, O C M, Han, J H, Staples, C R, Adesogan, A T
• Format: Journal Article
• Language: English
• Published: United States 01-10-2012
• Subjects: Aflatoxin B1 - adverse effects; Aflatoxin B1 - pharmacokinetics; Aflatoxin M1 - analysis; Animal Feed - adverse effects; Animals; Bentonite - pharmacology; Bentonite - therapeutic use; Cattle; Diet - veterinary; Fats - analysis; Female; Food Contamination; Haptoglobins - analysis; Immunity, Innate - drug effects; Immunity, Innate - physiology; Milk - chemistry; Milk Proteins - analysis
• ISSN: 1525-3198
• DOI: 10.3168/jds.2011-5287

This project aimed to examine the effects of adding 2 doses of a montmorillonite-based mycotoxin adsorbent on milk aflatoxin M(1) (AFM(1)) concentrations and the performance and innate immune response of dairy cows fed an aflatoxin B(1) (AFB(1))-contaminated diet. Eight lactating cows were used in a duplicated 4×4 Latin square design with 12-d periods. Treatments included the following: (1) control diet (C), (2) aflatoxin diet (T) containing C and 75 µg of AFB(1)/kg, 3) low-clay (LC) diet containing T and Calibrin A (Amlan International, Chicago, IL) added at 0.2% of the diet dry matter (DM), and 4) high-clay diet (HC) containing T and Calibrin A added at 1% of the diet DM. Milk production and DM intake were recorded daily and milk was sampled twice daily on d 5, 9, 10, 11, and 12 in each period. Blood samples were collected on d 5 and 9 of each period. Dietary treatments did not affect DM intake, milk yield, or feed efficiency. Even though cows were limit fed, feeding T instead of C reduced milk fat yield (0.67 vs. 0.74 kg/d) and milk protein concentration (3.28 vs. 3.36%). Concentrations of AFM(1) in milk of cows fed the T and LC diets were similar (0.57 and 0.64 µg/kg) and greater than those of cows fed the HC diet (0.46 µg/kg). Haptoglobin concentration was greater (22.0 vs. 14.4) and β(2)-integrin expression (220 vs. 131) tended to be greater in cows fed diet T instead of C, but values for cows fed LC, HC, and C did not differ. In comparison to C, feeding T increased the innate immune response and decreased milk fat yield and milk protein concentration, but feeding LC and HC did not affect these measures. Only the HC diet reduced milk AFM(1) concentration.