Enzyme production by solid‐state fermentation on soybean meal: A comparative study of conventional and ultrasound‐assisted extraction methods

• Published in: Biotechnology and applied biochemistry Vol. 66; no. 3; pp. 361 - 368
• Main Authors: Salim, Abdalla Ali, Grbavčić, Sanja, Šekuljica, Nataša, Vukašinović‐Sekulić, Maja, Jovanović, Jelena, Jakovetić Tanasković, Sonja, Luković, Nevena, Knežević‐Jugović, Zorica
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-05-2019
• Subjects: Agricultural production; alpha-Amylases - biosynthesis; alpha-Amylases - isolation & purification; alpha-Amylases - metabolism; Amylases; Bacillus subtilis; Bacillus subtilis - enzymology; Cellulase; Cellulase - biosynthesis; Cellulase - isolation & purification; Cellulase - metabolism; Comparative studies; Crop yield; Enzymes; extraction; Fermentation; Fermented food; Glycine max - chemistry; Glycine max - metabolism; Peptide Hydrolases - biosynthesis; Peptide Hydrolases - isolation & purification; Peptide Hydrolases - metabolism; protease; Solid state fermentation; Soybeans; Substrates; Ultrasonic imaging; Ultrasonic testing; Ultrasonic Waves; Ultrasound; α-Amylase; protease; α-amylase; Bacillus subtilis; cellulase; extraction; solid-state fermentation; ultrasound
• ISSN: 0885-4513; 1470-8744
• DOI: 10.1002/bab.1732

Protease, cellulase, and α‐amylase producing Bacillus subtilis strain was cultivated by solid‐state fermentation technique using soybean meal as a substrate. The aim of the present study was to establish a highly efficient enzymes’ extraction method as a first stage in downstream processing. The conventional extraction procedure was optimized by determining pH, stirring rate, solid/liquid ratio, and time of extraction on enzymes’ recoveries from fermented soybean meal. Yields of leached enzymes were compared to the amounts of enzymes that are achieved with ultrasound‐assisted extraction (UAE). UAE was established to be superior method for obtaining higher yields of proteases (up to 330 IU) and α‐amylases (825 IU), under significantly shorter extraction time and gaining more concentrated product. However, the obtained model predicts that conventional process led to a product with a higher cellulolytic activity (≥7.5 IU).