Involvement of the RpoN protein in the transcription of the oprE gene in Pseudomonas aeruginosa

• Published in: FEMS microbiology letters Vol. 162; no. 1; pp. 31 - 37
• Main Authors: Yamano, Yoshinori, Nishikawa, Tohru, Komatsu, Yoshihide
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-05-1998; Blackwell; Oxford University Press
• Subjects: Aerobic conditions; Aerobiosis; Anaerobic conditions; Anaerobiosis; Bacterial Proteins; Bacteriology; Base Sequence; Binding sites; Biological and medical sciences; DNA-Binding Proteins; DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - genetics; DNA-Directed RNA Polymerases - physiology; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Bacterial - genetics; Genes, Bacterial - genetics; Genetics; Integration host factor; Membrane proteins; Microbiology; Molecular Sequence Data; Mutation; Nucleotides; OprE; Porin; Porins - genetics; Promoter Regions, Genetic - genetics; Proteins; Pseudomonas aeruginosa; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - metabolism; Regulatory proteins; RNA polymerase; RNA Polymerase Sigma 54; RpoN; RpoN protein; Sigma factor; Sigma Factor - genetics; Sigma Factor - physiology; Transcription; Transcription, Genetic - genetics; OprE; Porin; RpoN; Pseudomonadales; Membrane protein; Regulation(control); Transcription; Initiation factor σ; Bacteria; Pseudomonadaceae; Pseudomonas aeruginosa; Gene expression
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.1998.tb12975.x

Abstract
OprE is a channel-forming outer membrane protein of Pseudomonas aeruginosa, the expression of which is induced under anaerobic conditions. We constructed various mutants and observed the effects on oprE expression. Deficiency in RpoN, an alternative sigma factor for RNA polymerase, abolished oprE expression under aerobic conditions, but did not affect the expression under anaerobic conditions. One mutation on the putative RpoN recognition site also caused reduction of oprE expression. The region 500 nucleotides upstream of the mRNA start site was required for optimal oprE transcription, which contains an AT-rich region including a putative integration host factor binding site. These results indicate that OprE production is directly or indirectly controlled by RpoN but also require some other regulatory proteins bound to the upstream region.