Ultrastructural detection of kinetochores by silver impregnation

We describe a simple silver impregnation method for the ultrastructural detection of kinetochores on meiotic chromosomes of the grasshopper Eyprepocnemis plorans. Testes were fixed with glutaraldehyde and silver-impregnated. After Epon 812 embedding, ultrathin cutting and counterstaining with uranyl...

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Bibliographic Details
Published in:	Chromosome research Vol. 2; no. 5; pp. 369 - 375
Main Authors:	Rufas, J S, Mazzella, C, García de la Vega, C, Suja, J A
Format:	Journal Article
Language:	English
Published:	Netherlands 01-09-1994
Subjects:	Animals Grasshoppers - genetics Kinetochores - ultrastructure Male Meiosis Silver Staining Spermatocytes - ultrastructure
Online Access:	Get full text
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Summary:	We describe a simple silver impregnation method for the ultrastructural detection of kinetochores on meiotic chromosomes of the grasshopper Eyprepocnemis plorans. Testes were fixed with glutaraldehyde and silver-impregnated. After Epon 812 embedding, ultrathin cutting and counterstaining with uranyl acetate, sections were studied by transmission electron microscopy. The meiotic chromosomes showed differentially silver-impregnated 'ball and cup' kinetochores. Some pericentriolar material also showed silver deposits. These observations are discussed in the light of previous results obtained by light microscopy of silver-stained spermatocytes in which both kinetochores and pericentriolar material were also preferentially stained. These results suggest a role for acidic proteins in the composition of these structures.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0967-3849 1573-6849
DOI:	10.1007/bf01552796