Uptake pathway for Ag bioaccumulation in three benthic invertebrates exposed to contaminated sediments

• Published in: Marine ecology. Progress series (Halstenbek) Vol. 270; pp. 141 - 152
• Main Authors: Yoo, Hoon, Lee, Jung-Suk, Lee, Byeong-Gweon, Lee, In Tae, Schlekat, Chris E., Koh, Chul-Hwan, Luoma, Samuel N.
• Format: Journal Article
• Language: English
• Published: Oldendorf Inter-Research 14-04-2004
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Bioaccumulation; Bioavailability; Biological and medical sciences; Clams; Diet; Food; Food rationing; Fundamental and applied biological sciences. Psychology; Leptocheirus plumulosus; Macoma balthica; Neanthes arenaceodentata; Sea water; Sea water ecosystems; Sediments; Silver; Sulfides; Synecology; Uptake route; Ecotoxicology; Leptocheirus plumulosus; Toxicity; Neanthes arenaceodentata; Sediments; Uptake; Marine environment; Sulfides; Silver; Acid-volatile sulfides; Bivalvia; Bioaccumulation; Macoma balthica; Mollusca; Biological accumulation; Invertebrata
• ISSN: 0171-8630; 1616-1599
• DOI: 10.3354/meps270141

We exposed 3 benthic invertebrates, the clamMacoma balthica, the polychaeteNeanthes arenaceodentataand the amphipodLeptocheirus plumulosus, to Ag-contaminated sediments to evaluate the relative importance of various uptake routes (sediments, porewater or overlying water, and supplementary food) for Ag bioaccumulation. Silver bioaccumulation was evaluated at 4 levels of sediment Ag (0.1, 0,3, 1,2 and 3.3 μmol Ag g–1) and 2 levels of acid-volatile sulfide (AVS), <0.5 or ~40 μmol g–1, and compared among food treatments with or without Ag contamination, or with different food rations.L. plumulosuswere incubated for 35 d in the Ag-contaminated sediments after 3 mo of Ag-sediment equilibration, andM. balthicaandN. arenaceodentatafor 19 d after 5 mo equilibration. Ag bioaccumulation in the 3 organisms was significantly correlated with 1N HCl-extractable Ag concentrations (Ag-SEM: simultaneously extracted Ag with AVS) in sediments. The Ag concentrations in porewater and overlying water were greatest in the sediments with least AVS, consistent with previous studies. Nevertheless, the amphipod and clam exposed to oxic sediments (<0.5 μmol AVS g–1) accumulated amounts of Ag similar to those accumulated by organisms exposed to anoxic sediments (~40 μmol AVS g–1), when Ag-SEM levels were comparable. The dissolved Ag source was important for bioaccumulation in the polychaeteN. arenaceodentata. Amphipods fed Ag-contaminated food contained ~1.8-fold more tissue Ag concentrations than those fed uncontaminated food. As suggested in kinetic (DYMBAM) modeling studies, ingestion of contaminated sediments and food were the principle routes of Ag bioaccumulation by the benthic invertebrates during chronic exposure, but the relative importance of each uptake route differed among species.