Identification of molecular markers linked to head smut resistance gene (Shs) in sorghum by RFLP and RAPD analyses

• Published in: Phytopathology Vol. 84; no. 8; pp. 830 - 833
• Main Authors: Oh, B.J. (Texas AandM University, College Station), Frederiksen, R.A, Magill, C.W
• Format: Journal Article Conference Proceeding
• Language: English
• Published: St. Paul, MN American Phytopathological Society 1994
• Subjects: ADN; AGENT PATHOGENE; Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; CHAMPIGNON; EPREUVE SUR LA DESCENDANCE; Fundamental and applied biological sciences. Psychology; GENE; GENES; GENETICA; Genetics and breeding of economic plants; GENETIQUE; GENOTIPOS; GENOTYPE; HONGOS; LIGNEE CONSANGUINE; LINEAS CONSANGUINEAS; MARCADORES GENETICOS; MARQUEUR GENETIQUE; ORGANISMOS PATOGENOS; Pest resistance; Plant pathogens; POLIMORFISMO; POLYMORPHISME; PRUEBAS DE DESCENDENCIA; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; SORGHUM BICOLOR; SPHACELOTHECA; Sporisorium reilianum; Varietal selection. Specialized plant breeding, plant breeding aims; Biological properties; Hybrid variety; Monocotyledones; Genetic marker; Plant pathogen; Artificial selection; Basidiomycetes; Genetic character; Cereal crop; Fungi; Sorghum bicolor; Sphacelotheca reiliana; Gene; Gramineae; Angiospermae; Molecular probe; Locus; Nucleic acid; Thallophyta; Genetic mapping; Varietal selection; Fungus resistance; Segregation analysis; Restriction fragment length polymorphism; DNA; RAPD markers; Spermatophyta; Molecular biology; Genome
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-84-830

Restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) methods were used to find markers linked to a head smut resistance gene (Shs) in sorghum. To select parents for a mapping population, RFLPs were identified between four resistant accessions (Lahoma Sudan, White Kafir, SC325, and CS3541) and four susceptible accessions (RTx7078, SC170-6-17, BTx399, and BTx623) for three restriction enzymes (EcoRI EcoRV and HindIII) with 43 maize genomic clones. Since SC325 (resistant) and RTx7078 (susceptible) showed the maximum RFLP frequency, these accessions were selected for mapping. Fifty-two F2 progenies from a selfed cross between accessions SC325 and RTx7078 were used to map the Shs locus. One hundred twenty-four sorghum genomic clones with five restriction enzymes (BamHI EcoRI EcoRV HindIII, and XbaI) and 326 RAPD markers were used for linkage analysis with Shs. Linkage of RFLP and RAPD loci with Shs was verified by using F3 lines to determine the Shs genotypes of the F2 plants after inoculation with a race 5 isolate of Sporisorium reilianum. RFLP and RAPD analyses revealed that RFLP loci detected by probes pSbTXS560 and pSbTXS1294 and one RAPD locus from primer OPG5 were linked to Shs