Sensitive electrochemical detection of superoxide anion using gold nanoparticles distributed poly(methyl methacrylate)-polyaniline core-shell electrospun composite electrode

• Published in: Analyst (London) Vol. 136; no. 8; p. 1557
• Main Authors: Santhosh, Padmanabhan, Manesh, Kalayil Manian, Lee, Se-Hee, Uthayakumar, Sivaperumal, Gopalan, Anantha Iyengar, Lee, Kwang-Pill
• Format: Journal Article
• Language: English
• Published: England 21-04-2011
• Subjects: Aniline Compounds - chemistry; Biosensing Techniques - methods; Electrochemical Techniques - methods; Electrodes; Electron Transport; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Gold - chemistry; Metal Nanoparticles - chemistry; Nanofibers - chemistry; Polymethyl Methacrylate - chemistry; Superoxide Dismutase - chemistry; Superoxide Dismutase - metabolism; Superoxides - analysis
• ISSN: 1364-5528
• DOI: 10.1039/c0an00616e

In the present communication, a novel composite nanofibrous electrode is developed for the detection of superoxide anion (O(2)˙(-)) in phosphate buffered saline (PBS). The composite fiber electrode is fabricated by dispersing gold nanoparticles onto poly(methyl methacrylate) (PMMA)-polyaniline (PANI) core-shell electrospun nanofibers. The constructed architecture is proven to be a favorable environment for the immobilization of the enzyme, superoxide dismutase (SOD). Direct electron transfer is achieved between SOD and the electrode with an electron transfer rate constant of 8.93 s(-1). At an applied potential of +300 mV, PMMA/PANI-Au(nano)/SOD-ESCFM shows highly sensitive detection of O(2)˙(-). In addition to this, quantification of different activities of SOD is realized at PMMA/PANI-Au(nano)/SOD-ESCFM. These analytical features offer great potential for construction of the third-generation O(2)˙(-) biosensor.