In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation

• Published in: Plant biotechnology reports Vol. 5; no. 1; pp. 35 - 43
• Main Authors: Negi, Divya, Saxena, Sanjay
• Format: Journal Article
• Language: English
• Published: Japan Springer Japan 2011; Springer Nature B.V; 한국식물생명공학회
• Subjects: Acetic acid; Acids; Agriculture; Analysis; Biomedical and Life Sciences; Biotechnology; Cell Biology; Experiments; Flowers & plants; In vitro fertilization; Life Sciences; Original Article; Plant Biochemistry; Plant propagation; Plant Sciences; Protocol; Seeds; Studies; Sucrose; 농학; Tissue culture; ISSR; Axillary shoot proliferation; Rhizome
• ISSN: 1863-5466; 1863-5474
• DOI: 10.1007/s11816-010-0154-z

This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of
Bambusa nutans
. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with 4.4 μM benzylaminopurine (BA) and 2.32 μM kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% bud-break. The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with 13.2 μM BA, 2.32 μM Kin, and 0.98 μM indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted with 100% success on half-strength MS liquid medium supplemented with 9.8 μM IBA, 2.85 μM indole-3-acetic acid (IAA), 2.68 μM naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages.