Anti-Inflammatory and Physicochemical Characterization of the Croton rhamnifolioides Essential Oil Inclusion Complex in β-Cyclodextrin

• Published in: Biology (Basel, Switzerland) Vol. 9; no. 6; p. 114
• Main Authors: Oliveira Brito Pereira Bezerra Martins, Anita, Wanderley, Almir Gonçalves, Alcântara, Isabel Sousa, Rodrigues, Lindaiane Bezerra, Cesário, Francisco Rafael Alves Santana, Correia de Oliveira, Maria Rayane, Castro, Fyama Ferreira e, Albuquerque, Thaís Rodrigues de, da Silva, Maria Sanadia Alexandre, Ribeiro-Filho, Jaime, Coutinho, Henrique Douglas Melo, Menezes, Paula Passos, Quintans-Júnior, Lucindo José, Araújo, Adriano Antunes de Souza, Iriti, Marcello, Almeida, Jackson Roberto Guedes da Silva, Menezes, Irwin Rose Alencar de
• Format: Journal Article
• Language: English
• Published: MDPI 30-05-2020; MDPI AG
• Subjects: Croton rhamnifolioides; cyclodextrin; inflammation
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology9060114

Croton rhamnifolioides is used in popular medicine for the treatment of inflammatory diseases. The objective of this study was to characterize and evaluate the anti-inflammatory effect of C. rhamnifolioides essential oil complexed in β-cyclodextrin (COEFC). The physicochemical characterization of the complexes was performed using different physical methods. The anti-inflammatory activity was evaluated in vivo by ear edema, paw edema, cotton pellet-induced granuloma, and vascular permeability by Evans blue extravasation. The mechanism of action was validated by molecular docking of the major constituent into the cyclooxygenase-2 (COX-2 enzyme). All doses of the COEFC reduced acute paw edema induced by carrageenan and dextran, as well as vascular permeability. Our results suggest the lowest effective dose of all samples inhibited the response induced by histamine or arachidonic acid as well as the granuloma formation. The complexation process showed that the pharmacological effects were maintained, however, showing similar results using much lower doses. The results demonstrated an involvement of the inhibition of pathways dependent on eicosanoids and histamine. Complexation of β-cyclodextrin/Essential oil (β-CD/EO) may present an important tool in the study of new compounds for the development of anti-inflammatory drugs.