Micropropagation of Physalis species with economic potential

• Published in: Revista Ceres Vol. 68; no. 6; pp. 521 - 529
• Main Authors: Silva, Luciana Sabini Da, Villa, Fabíola, Silva, Daniel Fernandes Da, Silva, Edvan Costa Da, Ritter, Giovana, Eberling, Tatiane
• Format: Journal Article
• Language: English
• Published: Vicosa Universidade Federal de Viçosa-UFV, Revista Ceres 01-12-2021; Universidade Federal de Viçosa; Universidade Federal De Viçosa
• Subjects: AGRICULTURE, MULTIDISCIPLINARY; Asepsis; Autoclaving; Crop production; Culture media; Distilled water; Experiments; Explants; Fruits; Genetic variability; Germination; in vitro culture; Laboratories; Micropropagation; Multiplication; Orchards; Physalis sp; Plantlets; Polymer films; Polyvinyl chloride; Propagation; Seeds; Sucrose; culture media; sucrose; asepsis; Physalis sp; in vitro culture
• ISSN: 0034-737X; 2177-3491; 2177-3491
• DOI: 10.1590/0034-737x202168060003

Because it has high added value, fruit production is an alternative for small products that constitute the highest income per production area (Negri et al., 2016). Asexual propagation is the main form of physalis multiplication, since this fruit has a large amount of seeds and a high germination rate, however it provides plantlets with high genetic variability, resulting in plants with different growth, vigor, performance and production of fruits, characteristics that are not interesting for commercial orchards (Oliveira et al., 2013). After the procedures, the seeds were washed four times in distilled water and autoclaved After the sanitization, 5 seeds were allocated by glass bottle, with a total capacity of 300 mL volum, containing 25 mL of media culture MS (Murashige & Skoog, 1962), added to 30 g L-1 of sucrose, 6 g L-1 of agar (Himedia(r)) and pH = 5,8, adjusted before autoclaving. After the autoclaving, they were sealed with plastic film of PVC, in order to avoid contaminations and kept in wooden shelves (45 x 30 cm), in a growth room, with photoperiod of 16 h light and temperature ± 24°C. In the experiments II, III and IV were used explants of pre-established plants in vitro from seeds were used.