Functional genomics of the murine immune system

• Published in: Immunologic research Vol. 23; no. 2-3; pp. 289 - 300
• Main Author: Ruley, H E
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01-01-2001
• Subjects: Animals; B-Lymphocytes - immunology; DNA Methylation; DNA Repair; Embryo, Mammalian - cytology; Expressed Sequence Tags; Gene Expression Regulation; Gene Library; Gene Targeting; Genes; Genes, Lethal; Genes, Recessive; Genes, Synthetic; Genetic Vectors - genetics; Genetics; Genomics; Heterogeneous-Nuclear Ribonucleoproteins; Humans; Immune System; Inflammation Mediators; Leukopenia - genetics; Lymphocyte Activation - genetics; Mammalia; Mice - embryology; Mice - genetics; Mice - immunology; Mutagenesis; Mutagenesis, Site-Directed; Phenotype; Proviruses - genetics; Retroviridae - genetics; Ribonucleoproteins - deficiency; Ribonucleoproteins - genetics; Ribonucleoproteins - physiology; Ribonucleoproteins, Small Nucleolar - genetics; RNA Splicing; Rodents; Species Specificity; Stem Cells
• ISSN: 0257-277X; 0257-277X; 1559-0755
• DOI: 10.1385/IR:23:2-3:289

The mouse and human genome sequences provide new opportunities to characterize mammalian gene functions on a genome-wide level. Toward this end, we have developed strategies for tagged-sequence mutagenesis in mice. Tagged-sequence mutagenesis has been used first, to analyze genes implicated in posttranscriptional gene regulation, and second, to identify genes important in immune cell development and function.