MyC Factor Analogue CO5 Promotes the Growth of Lotus japonicus and Enhances Stress Resistance by Activating the Expression of Relevant Genes

• Published in: Journal of fungi (Basel) Vol. 10; no. 7; p. 458
• Main Authors: Luo, Xinhao, Jiang, Jiaqing, Zhou, Jing, Chen, Jin, Cheng, Beijiu, Li, Xiaoyu
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28-06-2024
• Subjects: Agricultural production; Agriculture; arbuscular mycorrhizal fungi; Arbuscular mycorrhizas; Beans; chitin oligomer 5; Colonization; Fungi; Gene expression; Genes; Genetic research; Growth; hydroponic system; Hydroponics; Legumes; Lotus japonicus; Mimosaceae; Myc protein; Plant growth; Salinity; Seeds; Symbiosis; Temperature; China; Beijing China; chitin oligomer 5; hydroponic system; arbuscular mycorrhizal fungi; Lotus japonicus
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof10070458

The symbiotic relationship between arbuscular mycorrhizal fungi (AMF) and plants is well known for its benefits in enhancing plant growth and stress resistance. Research on whether key components of the AMF colonization process, such as MyC factors, can be directly utilized to activate plant symbiotic pathways and key functional gene expression is still lacking. In this paper, we found that, using a hydroponics system with
, MyC factor analogue chitin oligomer 5 (CO5) had a more pronounced growth-promoting effect compared to symbiosis with AMF at the optimal concentration. Additionally, CO5 significantly enhanced the resistance of
to various environmental stresses. The addition of CO5 activated symbiosis, nutrient absorption, and stress-related signaling pathways, like AMF symbiosis, and CO5 also activated a higher and more extensive gene expression profile compared to AMF colonization. Overall, the study demonstrated that the addition of MyC factor analogue CO5, by activating relevant pathways, had a superior effect on promoting plant growth and enhancing stress resistance compared to colonization by AMF. These findings suggest that utilizing MyC factor analogues like CO5 could be a promising alternative to traditional AMF colonization methods in enhancing plant growth and stress tolerance in agriculture.