The relationships of concentrations of insulin, intact proinsulin and 32-33 split proinsulin with cardiovascular risk factors in type 2 (non-insulin-dependent) diabetic subjects

• Published in: Diabetologia Vol. 33; no. 9; pp. 532 - 537
• Main Authors: NAGI, D. K, HENDRA, T. J, RYLE, A. J, COOPER, T. M, TEMPLE, R. C, CLARK, P. M. S, SCHNEIDER, A. E, HALES, C. N, YUDKIN, J. S
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-09-1990
• Subjects: Adult; Aged; Anthropometry; Asia - ethnology; Biological and medical sciences; Cardiovascular Diseases - complications; Cholesterol - blood; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - physiopathology; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; England; European Continental Ancestry Group; Female; Humans; Insulin - blood; Male; Medical sciences; Middle Aged; Plasminogen Inactivators - analysis; Proinsulin - blood; Regression Analysis; Risk Factors; Triglycerides - blood; England; Asia; Endocrinopathy; Human; Enzyme; Cardiovascular disease; Plasminogen activator; Non insulin dependent diabetes; Insulin; Cholesterol; Concomitant disease; Concentration effect; Risk factor; Arterial pressure; Lipoprotein HDL
• ISSN: 0012-186X; 1432-0428
• DOI: 10.1007/BF00404140

Standard radioimmunoassay for insulin may substantially overestimate levels of insulin because of cross-reaction with other insulin-like molecules. We have measured concentrations of insulin, intact proinsulin and 32-33 split proinsulin using two-site monoclonal antibody based immunoradiometric assays, and of insulin by a standard radioimmunoassay ("immunoreactive insulin") in 51 Type 2 (non-insulin-dependent) diabetic subjects in the fasting state. The relationships of these concentrations were sought with those of total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, triglyceride, plasminogen activator inhibitor, blood pressure, and indices of body fat distribution. Significant relationships were apparent between concentrations of "immunoreactive insulin" as measured by standard radioimmunoassay and triglyceride (rs = 0.42, p less than 0.001), total cholesterol (rs = 0.25, p = 0.038), high density lipoprotein cholesterol (rs = -0.30, p = 0.018) and body mass index (rs = 0.30, p = 0.017), but only the relationships with triglyceride (rs = 0.36, p = 0.006) and body mass index (rs = 0.26, p = 0.34) remained significant when concentrations of immunoradiometrically measured insulin were employed. Concentrations of 32-33 split proinsulin, which comprises the major insulin-like molecule in these subjects, correlated positively with triglyceride (rs = 0.33, p = 0.009), total cholesterol (rs = 0.23, p = 0.050), and plasminogen activator inhibitor (rs = 0.26, p = 0.049), and negatively with high density lipoprotein cholesterol (rs = -0.29, p = 0.021).