Decolorization of Azo, Triphenylmethane and Anthraquinone Dyes by Laccase of a Newly Isolated Armillaria sp. F022

• Published in: Water, air, and soil pollution Vol. 223; no. 3; pp. 1045 - 1054
• Main Authors: Hadibarata, Tony, Yusoff, Abdull Rahim Mohd, Aris, Azmi, Salmiati, Hidayat, Topik, Kristanti, Risky Ayu
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-03-2012; Springer Nature B.V
• Subjects: Anthraquinone; Armillaria; Atmospheric Protection/Air Quality Control/Air Pollution; Azo dyes; Biosorption; Cell culture; Chemical industry; Climate Change/Climate Change Impacts; Decolorization; Dyes; Earth and Environmental Science; Effluents; Environment; Environmental monitoring; Enzymes; Fungi; Hydrogeology; Laccase; Lignin; Rainforests; Soil Science & Conservation; Studies; Textile industry; Tropical forests; Water Quality/Water Pollution; White rot; Brilliant Green; Laccase activity; Microbial decolorization; sp. F022; Reactive Black 5; Remazol Brilliant Blue R
• ISSN: 0049-6979; 1573-2932
• DOI: 10.1007/s11270-011-0922-6

A newly isolated white-rot fungus,
Armillaria
sp. strain F022, was isolated from the decayed wood in a tropical rain forest. Strain F022 was capable of decolorizing a variety of synthetic dyes, including azo, triphenylmethane, and anthraquinone dyes, with an optimal efficiency of decolorization obtained when dyes added after 96 h of culture, with the exception of Brilliant Green. All of the tested dyes were decolorized by the purified laccase in the absence of any redox mediators, but only a few were completely removed, while others were not completely removed even when decolorization time was increased. The laccase, with possible contributions from unknown enzymes, played a role in the decolorization process carried out by
Armillaria
sp. F022 cultures, and this biosorption contributed a negligible part to the decolorization by cultures. The effect of dye to fungal growth was also investigated. When dyes were added at 0 h of culture, the maximum dry mycelium weight (DMW) values in the medium containing Brilliant Green were 1/6 of that achieved by the control group. For other dyes, the DMW was similar with control. The toxic tolerance of dye for the cell beads was excellent at least up to a concentration of 500 mg/l. The optimum conditions for decolorization of three synthetic dyes are at pH 4 and 40°C.