Chemical composition, antioxidant and antibacterial activities and evaluation of cytotoxicity of the fractions obtained from Selaginella convoluta (Arn.) Spring (Selaginellaceae)

• Published in: Biotechnology, biotechnological equipment Vol. 32; no. 2; pp. 506 - 512
• Main Authors: Macêdo, Larissa Alves Ribeiro de Oliveira, Oliveira Júnior, Raimundo Gonçalves de, Souza, Grasielly Rocha, de Oliveira, Ana Paula, de Lavor, Érica Martins, Silva, Mariana Gama e, Pacheco, Alessandra Gomes Marques, de Menezes, Irwin Rose Alencar, Coutinho, Henrique Douglas Melo, Pessoa, Cláudia do Ó, da Costa, Marcília Pinheiro, Almeida, Jackson Roberto Guedes da Silva
• Format: Journal Article
• Language: English
• Published: Sofia Taylor & Francis Ltd 04-03-2018; Taylor & Francis Group
• Subjects: Acetic acid; Aluminum; Aluminum chloride; Anthracene; Antibacterial activity; antioxidant activity; Antioxidants; Bleaching; Carotene; Chemical composition; Chloroform; Colon; Cytotoxicity; Ethyl acetate; Evaluation; Flavonoids; Lignans; Linoleic acid; Phenolic compounds; Phenols; Scavenging; Selaginella; Selaginella convoluta; Steroid hormones; Steroids; Toxicity; Triterpenoids; β-Carotene
• ISSN: 1310-2818; 1314-3530
• DOI: 10.1080/13102818.2018.1431055

The aim of this study was to evaluate the chemical composition, antioxidant and antibacterial activities and cytotoxicity of fractions from Selaginella convoluta, obtained by liquid–liquid extraction using hexane (Sc-Hex), chloroform (Sc-CHCl3) and ethyl acetate (Sc-AcOEt). The phenolic and flavonoid contents were measured by the Folin-Ciocalteu and aluminium chloride methods, respectively. Antioxidant activities were evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and β-carotene-linoleic acid bleaching test. The antibacterial effect was evaluated by the method of microdilution and the cytotoxicity analysis in HCT-116 (colon), OVCAR-8 (ovarian) and SF-295 (brain) cells were carried out for MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) test. The fractions were positive for the presence of anthracene derivatives, flavonoids, lignans, naphthoquinones, steroids and triterpenoids. The Sc-Hex and Sc-AcOEt showed good antioxidant activities. The fractions of S. convoluta demonstrated antibacterial activity and showed weak cytotoxicity. These activities were correlated with presence of phenolic compounds in active fractions.