An alternative approach to conducting germination tests on chemically treated and untreated stored cotton seeds

The objective of this study was to assess the impact of incorporating vermiculite into germination tests for both chemically treated and untreated stored cotton seeds. To that end, two independent experiments were conducted using cotton seeds of the cultivar DP1746B2RF under chemical treatment with...

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Bibliographic Details
Published in:Journal of seed science Vol. 45
Main Authors: Cossa, Nasma Henriqueta Da Sorte, Carvalho, Everson Reis, Lima, Juliana Maria Espíndola, Moraes, Luiz Fernando de Souza, Miquicene, Felisberto Venâncio Chicamasso, Zevo, Ivan Natividade Júlio
Format: Journal Article
Language:English
Published: ABRATES - Associação Brasileira de Tecnologia de Sementes 01-01-2023
Associação Brasileira de Tecnologia de Sementes
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Summary:The objective of this study was to assess the impact of incorporating vermiculite into germination tests for both chemically treated and untreated stored cotton seeds. To that end, two independent experiments were conducted using cotton seeds of the cultivar DP1746B2RF under chemical treatment with a mixture of fungicide, insecticides, and nematicide; and both experiments were set up in a completely randomized design with four replications. In Experiment 1, the objective was to determine the ideal water volume for moistening the vermiculite. Four water volumes were used: 1.5, 2.0, 2.5, and 3.0 times the weight of the dry vermiculite. Experiment 2 was conducted in a 2 × 2 × 2 triple factorial arrangement consisting of two substrates (germitest paper and germitest paper + vermiculite), two treatments (chemically treated seeds and untreated seeds), and two storage periods (0 and 150 days). First germination count and germination were evaluated in the two experiments. From the results found in Experiment 1, the water volume corresponding to 3.0 times the weight of the vermiculite provided the best conditions for germination. In Experiment 2, the most appropriate substrate for conducting the germination test of untreated and chemically treated cotton seeds is vermiculite, which led to rapid germination and uniform seedling development. Resumo: Objetivou-se avaliar o efeito da inclusão da vermiculita no teste de germinação em sementes de algodão não tratadas e tratadas quimicamente. Para isso, foram conduzidos dois experimentos independentes, utilizando-se sementes de algodão da cultivar DP1746B2RF, submetidas ao tratamento químico com mistura de fungicida, inseticidas e nematicida, ambos, instalados em delineamento inteiramente casualizado com quatro repetições. No Experimento 1, objetivou-se determinar o volume de água ideal para o umedecimento da vermiculita. Foram utilizados quatro volumes de água: 1,5; 2,0; 2,5 e 3,0 vezes o peso da vermiculita seca. O Experimento 2 foi conduzido em esquema fatorial triplo 2 x 2 x 2 sendo: dois substratos (papel germitest e papel germitest + vermiculita), dois tratamentos (sementes tratadas quimicamente e sementes não tratadas) e dois períodos de armazenamento (0 e 150 dias). Avaliou-se nos dois experimentos, a primeira contagem de germinação e a germinação. Pelos resultados encontrados no Experimento 1, o volume de água correspondente a 3,0 vezes o peso da vermiculita proporcionou as melhores condições para germinação. No Experimento 2, o substrato mais apropriado para condução do teste de germinação de sementes de algodão não tratadas e tratadas quimicamente, é a vermiculita, tendo proporcionado rápida germinação e desenvolvimento uniforme das plântulas.
ISSN:2317-1537
2317-1545
2317-1545
DOI:10.1590/2317-1545v45266896