Somatic embryogenesis from mature leaves of rose (Rosa sp.)

• Published in: Plant cell reports Vol. 18; no. 6; pp. 467 - 472
• Main Authors: Kintzios, S, Manos, C, Makri, O
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-02-1999; Springer Nature B.V
• Subjects: 2,4-D; 6-benzyladenine; Acids; auxins; benzyladenine; Biological and medical sciences; Biotechnology; callus; Chlorophenoxyacetic acids; Cultivars; culture media; Developmental stages; embryo (plant); Embryonic growth stage; Embryos; Eukaryotic cell cultures; explants; Fundamental and applied biological sciences. Psychology; germination; gibberellic acid; Growth regulators; In vitro propagation: entire plant regeneration from tissues and cell cultures; indole acetic acid; indoleacetic acid; kinetin; Leaves; Methods. Procedures. Technologies; micropropagation; naphthaleneacetic acid; p-chlorophenoxyacetic acid; Plant cells and fungal cells; plant development; Plant growth; Rosa; somatic embryogenesis; stems; tissue culture; Embryonic development; Light effect; Vegetals; Induction; Essential oil plant; Rosaceae; Tissue culture; Explant; Genotype; Plant leaf; Rosa; Stem; Somatic embryo; Organogenesis; Dicotyledones; Angiospermae; Plant growth substance; Spermatophyta; Comparative study; Cultivar
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990050605

Several plant growth regulators (0.3-53.3 micromolars 6-benzyladenine, 2,4-dichlorophenoxyacetic acid, gibberellic acid, 3-indoleacetic acid, p-chlorophenoxyacetic acid, kinetin and alpha-naphthylacetic acid), alone or in combination, and culture conditions were tested for their capacity to induce somatic embryogenesis from mature leaf and stem explants of rose (Rosa sp.) of four commercial rose cultivars (Baccara, Mercedes, Ronto and Soraya). Somatic embryos were only induced from mature leaf explants derived from Soraya on Murashige and Skoog (MS) medium supplemented with 53.5 micromolars p-chlorophenoxyacetic acid and 4.6 micromolars kinetin, although satisfactory callus induction rates were obtained from all cultivars. After sub-culturing on the same medium, embryos at various developmental stages (globular, heart and torpedo shaped) were transferred for maturation onto a MS medium supplemented with 5.2 micromolars 6-benzyladenine and 5.7 micromolar 3-indoleacetic acid. Germination of mature embryos took place after subculturing them onto medium of the same composition. Plantlets regenerated from embryos and bearing three to four leaves were transferred to a greenhouse.