Localization of Legionella pneumophila in tissue using FITC-conjugated specific antibody and a background stain

Lightly staining formalin-fixed or fresh tissue with Gram's crystal violet obviates interfering nonspecific fluorescence by acting as a metachromatic stain in ultraviolet light. Against the easily recognized background of tissues and cells fluorescein isothiocyanate-tagged Legionella pneumophil...

Full description

Saved in:

Bibliographic Details
Published in:	American journal of clinical pathology Vol. 77; no. 5; p. 601
Main Authors:	Lowry, B S, Vega, Jr, F G, Hedlund, K W
Format:	Journal Article
Language:	English
Published:	England 01-05-1982
Subjects:	Animals Antibodies, Bacterial Fluorescein-5-isothiocyanate Fluoresceins Gentian Violet Guinea Pigs Legionella - immunology Legionella - isolation & purification Mice Mice, Inbred AKR Microscopy, Fluorescence Staining and Labeling Thiocyanates Ultraviolet Rays
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Lightly staining formalin-fixed or fresh tissue with Gram's crystal violet obviates interfering nonspecific fluorescence by acting as a metachromatic stain in ultraviolet light. Against the easily recognized background of tissues and cells fluorescein isothiocyanate-tagged Legionella pneumophila antibodies can then identify this bacterium in or on individual cells. This procedure can be run at room temperature in two hours and has the potential for further widespread applicability.
ISSN:	0002-9173
DOI:	10.1093/ajcp/77.5.601