The use of enzymatic preparation for the production of low molecular-weight chitosan from the king crab hepatopancrease

• Published in: Applied biochemistry and microbiology Vol. 45; no. 4; pp. 374 - 379
• Main Authors: Ilyina, A. V., Zagorskaya, D. S., Levov, A. N., Albulov, A. I., Kovacheva, N. P., Varlamov, V. P.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01-07-2009; Springer Nature B.V
• Subjects: Biochemistry; Bioengineering; Biomedical and Life Sciences; Crustaceans; Decapoda; Enzymes; Hydrolysis; Life Sciences; Marine; Medical Microbiology; Microbiology; Molecular weight; Chitinase; Chitin; Chitosan; Apply Biochemistry; Chitinase Activity
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683809040048

This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 700 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1: 200, 37°C, and 20 h duration of hydrolysis.