Lipin is required for efficient breakdown of the nuclear envelope in Caenorhabditis elegans

• Published in: Journal of cell science Vol. 122; no. 12; pp. 1963 - 1969
• Main Authors: Gorjánácz, Mátyás, Mattaj, Iain W
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Limited 15-06-2009
• Subjects: Animals; Animals, Genetically Modified; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins - physiology; Cell Nucleus Division - physiology; Endoplasmic Reticulum - physiology; Mitosis - physiology; Nuclear Envelope - metabolism; Nuclear Envelope - physiology; Nuclear Lamina - metabolism; Nuclear Lamina - physiology; Nuclear Proteins - physiology
• ISSN: 0021-9533; 1477-9137
• DOI: 10.1242/jcs.044750

The nuclear envelope (NE) is a double lipid bilayer that separates nucleus and cytoplasm. In metazoa, NE breakdown (NEBD) occurs during prophase and NE reformation around segregated chromatids occurs at anaphase-telophase. We identified Caenorhabditis elegans Lipin homologue (called Lpin-1) as an essential factor with roles in endoplasmic reticulum (ER) organization and NEBD. RNAi-mediated downregulation of Lpin-1 had no effect on timely entry into mitosis or on the early steps of NEBD, but Lpin-1 was required for disassembly of the nuclear lamina during late NEBD. This Lpin-1 requirement appears to be separable from the effect of Lpin-1 on the peripheral ER.