Detection of Oropouche virus segment S in patients and inCulex quinquefasciatus in the state of Mato Grosso, Brazil

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 110; no. 6; pp. 745 - 754
• Main Authors: Cardoso, Belgath Fernandes, Serra, Otacília Pereira, Heinen, Letícia Borges da Silva, Zuchi, Nayara, Souza, Victor Costa de, Naveca, Felipe Gomes, Santos, Marcelo Adriano Mendes dos, Slhessarenko, Renata Dezengrini
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Oswaldo Cruz, Ministério da Saúde 01-09-2015; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Adolescent; Adult; Aged; Animal Distribution; Animals; Base Sequence; Brazil - epidemiology; Bunyaviridae Infections - blood; Bunyaviridae Infections - epidemiology; Chlorocebus aethiops; Culex - classification; Culex - virology; Dengue - epidemiology; Disease Outbreaks; Female; Fever - physiopathology; Fever - virology; Genotype; Humans; Male; Mato Grosso; Middle Aged; mosquitoes; OROV; Orthobunyavirus; Orthobunyavirus - classification; Orthobunyavirus - genetics; PARASITOLOGY; phylogenetic analysis; Phylogeny; Polymerase Chain Reaction; Prevalence; RNA, Viral - isolation & purification; Serogroup; Simbu virus - classification; Simbu virus - genetics; TROPICAL MEDICINE; Vero Cells; Young Adult; Brazil; Mato Grosso; mosquitoes; Orthobunyavirus; phylogenetic analysis; OROV
• ISSN: 0074-0276; 1678-8060; 1678-8060; 0074-0276
• DOI: 10.1590/0074-02760150123

This study aimed to investigate the circulation of Orthobunyavirus species in the state of Mato Grosso (MT) Brazil. During a dengue outbreak in 2011/2012, 529 serum samples were collected from patients with acute febrile illness with symptoms for up to five days and 387 pools of female Culex quinquefasciatus captured in 2013 were subjected to nested-reverse transcription-polymerase chain reaction for segment S of the Simbu serogroup followed by nucleotide sequencing and virus isolation in Vero cells. Patients (5/529; 0.9%) from Cuiabá (n = 3), Várzea Grande (n = 1) and Nova Mutum (n = 1) municipalities were positive for the S segment of Oropouche virus (OROV). Additionally, eight/387 Cx. quinquefasciatus pools were positive for the segment, with a minimum infection rate of 2.3. Phylogenetic analysis indicated that all the samples belong to the subgenotype Ia, presenting high homology with OROV strains obtained from humans and animals in the Brazilian Amazon. The present paper reports the first detection of an Orthobunyavirus, possibly OROV, in patients and in Cx. quinquefasciatus mosquitoes in MT. This finding reinforces the notion that arboviruses frequently reported in the Amazon Region circulate sporadically in MT during dengue outbreaks.