Identification and characterization of phosphoenolpyruvate: fructose phosphotransferase systems in three Streptomyces species

• Published in: Microbiology (Society for General Microbiology) Vol. 141; no. 1; pp. 51 - 58
• Main Authors: Titgemeyer, Friedrich, Walkenhorst, Jurgen, Reizer, Jonathan, Stuiver, Maarten H, Cui, Xuewen, Saier, Milton H., Jr
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-01-1995; Society for General Microbiology
• Subjects: Bacillus subtilis - enzymology; Bacteriology; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Fructose - metabolism; Fundamental and applied biological sciences. Psychology; Genetic Complementation Test; Hydrogen-Ion Concentration; Kinetics; Microbiology; Mission oriented research; Permeability, membrane transport, intracellular transport; Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism; Phosphorylation; Physiology and metabolism; Species Specificity; Streptomyces - enzymology; Streptomyces - genetics; Streptomyces coelicolor; Streptomyces lividans; Phosphorylation; Streptomycetaceae; Enzyme; Streptomyces coelicolor; Phosphoenolpyruvate-dependent phosphotransferase system; Fructose; Actinomycetales; Regulation(control); Enzymatic activity; Membrane transport; Streptomyces lividans; Bacteria; Actinomycetes; Carbohydrate
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-141-1-51

Department of Biology, University of California at San Diego, La Jolla, CA 92093-0116, USA
*Milton H. Saier, Jr. Tel: [phone]. Fax: [phone]. e-mail: [e-mail]
ABSTRACT
SUMMARY: Streptomyces lividans, S. coelicolor and S. griseofuscus were examined for the presence of the enzymes of the phosphoenolpyruvate:sugar phosphotransferase system (PTS). All three species were shown to possess Enzyme I, HPr and fructose-specific Enzyme II (II Fru ) activities. In S. lividans and S. coelicolor, all three PTS enzymes were fructose-inducible, but in S. griseofuscus the system was expressed constitutively. These organisms apparently lack the HPr(Ser) kinase and HPr(Ser-P) phosphatase that characterize low-GC Gram-positive bacteria.
Keywords: sugar transport, phosphorylation, phosphotransferase system, Streptomyces, regulation