Effect of sulfated galactan from Porphyra haitanensis on H2O2-induced premature senescence in WI-38 cells

• Published in: International journal of biological macromolecules Vol. 106; pp. 1235 - 1239
• Main Authors: Zhang, Zhongshan, Wang, Xiaomei, Su, Huili, Pan, Yongliang, Han, Jianfang, Zhang, Taisheng, Mao, Genxiang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-01-2018
• Subjects: Antioxidant activities; Cellular senescence; Sulfated galactan; Antioxidant activities; Cellular senescence; Sulfated galactan
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.08.123

Porphyran sulfated galactan extracted from red algae Porphyra haitanensis is a sulfated polysaccharide, which possesses excellent activities. In the present study, WI-38 cells were treated with H2O2 to induce premature senescence and then the protection of porphyran against aging in vitro and associated molecular mechanisms were investigated. The protection occurred in a dose-dependent manner, offering an optimal efficacy starting at 10μg/mL. The proportion of SA-β-gal positive cells in porphyran group decreases from 53% to 23% in the cultures at 30 PDs. Porphyran has been detected specifically reducing SAHF-like foci formation in senescent cells. In addition, porphyran significantly affected the p53-p21 pathways in H2O2-treated WI-38 cells. Our data suggest the promising role of porphyran as an attractive and bio-safe agent with the potential to retard senescence and attenuate senescence-related diseases.