Production of gold nanoparticles by biogenesis using bacteria

• Published in: Microbiology (New York) Vol. 85; no. 1; pp. 63 - 70
• Main Authors: Radtsig, M. A, Koksharova, O. A, Nadtochenko, V. A, Khmel’, I. A
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 2016
• Subjects: Anabaena; Azotobacter; Azotobacter chroococcum; Azotobacter vinelandii; Bacteria; biogenesis; Biomedical and Life Sciences; Cyanobacteria; Experimental Articles; gold; Life Sciences; Medical Microbiology; Microbiology; nanogold; nitrogen fixation; nitrogen-fixing bacteria; nitrogen-fixing cyanobacteria; Nostoc; salts; Synechococcus; Synechocystis; biogenesis; nitrogen fixation; gold nanoparticles; cyanobacteria
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261716010094

Diazotrophic cyanobacteria Anabaena sp. PCC 7120, four Nostoc strains, and two Azotobacter species (A. vinelandii and A. chroococcum) were found to produce gold nanoparticles (GNP) under nitrogen fixation conditions. GNP biogenesis occurred at AuHCl₄ concentrations from 0.1 to 1 mM. In the cultures of unicellular cyanobacteria Synechococcus sp. PCC 7942 and Synechocystis sp. PCC 6803 incapable of nitrogen fixation, no GNP were formed at the same concentrations of gold salts. The plasmon resonance band peak was located at 552 nm. This position is characteristic of spherical GNP 10 to 30 nm in size. Small amounts of GNP were also formed in the culture liquid supernatants of the tested nitrogen-fixing bacteria at AuHCl₄ concentrations from 0.25 to 0.5 mM.