Insect cell growth evaluation during serum-free adaptation in stirred suspension cultures

• Published in: Biotechnology techniques Vol. 11; no. 2; pp. 117 - 120
• Main Authors: Cruz, P.E, Moreira, J.L, Carrondo, M.J.T
• Format: Journal Article
• Language: English
• Published: London Chapman and Hall 1997
• Subjects: Animal cells; Biological and medical sciences; Biotechnology; Establishment of new cell lines, improvement of cultural methods, mass cultures; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Spodoptera frugiperda; Culture medium; Cell proliferation; Cell culture; Serum free medium; Agitation; Growth rate; Insecta; Spodoptera frugiperda; Thawing; Freezing; Suspension culture; Cell line; Arthropoda; Animal; Lepidoptera; Noctuidae; Invertebrata; Adaptation
• ISSN: 0951-208X; 1573-6784
• DOI: 10.1023/B:BITE.0000034015.83434.66

Sf-9 insect cells were adapted to three different serum-free media 9SF900II, EXCELL 401 and IPL/41 supplemented) in 125 ml stirred vessels by gradually reducing serum concentration from 10 to 0% (v /v). TC100 medium supplemented with 10% fetal bovine serum was used as control. With this procedure it was possible to obtain cells fully adapted to SF900II and EXCELL 401 in 5 weeks. The adapted cells could be frozen in serum-free medium and thawed without any decrease in specific growth rate or maximum cell concentration. Even after 4 months of culture in stirred vessels at 170 rpm the specific growth rate and maximum cell concentration (0.031/h and 4.8 x 10 super(6) cells/ml, respectively) remained constant.