Multiplex Human Immunodeficiency Virus 1/2 and Hepatitis C Virus Antibodies Simultaneous Detection Using Three-Dimensional Sol-Gel Nanoporous Capturing Technology

• Published in: Bulletin of the Korean Chemical Society Vol. 37; no. 12; pp. 1914 - 1919
• Main Authors: Hwang, Kyung Hoon, Ki, Yeona, Lee, Young Mi, Lee, Jin Ah, Choi, In Kyoung, Park, Jun Sung, Kim, Jinhong, Kang, Jasmine, Kukushkin, Yury, Oh, Eun Jee, Han, Kyung Ja, Kim, Soyoun
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag GmbH & Co. KGaA 01-12-2016; Wiley‐VCH Verlag GmbH & Co. KGaA; 대한화학회
• Subjects: Clinical trials; Hepatitis C virus; Human immunodeficiency virus; Multiplex diagnosis; Sol-gel capturing technology; 화학
• ISSN: 1229-5949; 0253-2964; 1229-5949
• DOI: 10.1002/bkcs.10969

The sol–gel nanoporous capturing technology represents a powerful approach where the sol–gel matrix constrains the motion of the encapsulated biomolecules (proteins, peptides, chemicals, antibodies, nucleotides, etc.) without physical adsorption or any modifications. This technology can be applied to multiplex immunoassay platform because several disease markers can be immobilized and tested at once. In this study, we have tested several thousand specimens using Hi3‐1 Multiplex human immunodeficiency virus (HIV) 1/2 and hepatitis C virus (HCV) antibody detection kit at two clinical trials institutes. These results showed a highest sensitivity (100%, n = 500 HIV‐positive specimens and n = 400 HCV‐positive) and specificity (99.99% for HIV and 99.83% for HCV, n = 7706 negative specimens) by using Hi3‐1 kit, which screens HIV1/2 and HCV antibodies simultaneously. Finally, we suggested that our screening technology was successfully utilized as a multiplex HIV and HCV diagnostic tool for blood bank screening.