In Vitro Development of a New Sponge-Based Delivery System for Intracanal Antimicrobial Administration in Endodontic Treatment

• Published in: Journal of clinical medicine Vol. 10; no. 12; p. 2725
• Main Authors: Manuel, Ruíz-Piñón, Tania, Gancedo-Gancedo, Rafael, Seoane-Prado, Antonio, Pérez-Estévez, José, Blanco-Méndez, Asteria, Luzardo-Álvarez, Pablo, Castelo-Baz, Alejandro, Lorenzo-Pouso, Pablo, Álvarez-Novoa, Benjamín, Martín-Biedma
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 21-06-2021; MDPI
• Subjects: Acids; Antibiotics; Antimicrobial agents; Biofilms; Clinical medicine; Collagen; Endodontics; Pathogens; Potassium; Teeth; Italy; St Louis Missouri; United States > US; Switzerland; Germany
• ISSN: 2077-0383; 2077-0383
• DOI: 10.3390/jcm10122725

This study aimed to evaluate the in vitro performance of collagen-based sponges as a drug delivery system for intracanal antimicrobial administration. Four groups of loaded collagen-based sponges (A, 0.3% w/v amoxicillin trihydrate: potassium clavulanate (4:1); B, 0.03% w/v chlorhexidine gluconate [CHX]; C, 0.3% w/v amoxicillin trihydrate: potassium clavulanate (4:1) and 0.03% w/v CHX; D, 1% w/v amoxicillin trihydrate: potassium clavulanate (4:1) and 0.03% w/v CHX) were designed. Release kinetics were tested in vitro on cultures in Petri dishes, and the effect on bacterial biofilms was studied ex vivo on 114 extracted human single-rooted teeth. Biofilm formation was tested by scanning electron microscopy (SEM). Collagen sponges containing amoxicillin and chlorhexidine showed a time-sustained antimicrobial effect in vitro and were also able to destroy mature biofilms ex vivo. This datum was validated by means of SEM-based study of E. faecalis and S. aureus biofilms.