One-step synthesis of thermally stable artificial multienzyme cascade system for efficient enzymatic electrochemical detection

• Published in: Nano research Vol. 12; no. 12; pp. 3031 - 3036
• Main Authors: Cheng, Xiqing, Zhou, Jinhong, Chen, Jiayu, Xie, Zhaoxiong, Kuang, Qin, Zheng, Lansun
• Format: Journal Article
• Language: English
• Published: Beijing Tsinghua University Press 01-12-2019; Springer Nature B.V
• Subjects: Atomic/Molecular Structure and Spectra; Biomedicine; Biomimetics; Biomolecules; Biotechnology; Cascade chemical reactions; Catalysis; Catalytic activity; Chemistry and Materials Science; Chemoreception; Condensed Matter Physics; Copper; Electrochemical analysis; Electrochemistry; Electrodes; Enzymes; Glucose oxidase; High temperature; Immobilization; Materials Science; Metal foams; Metal-organic frameworks; Mineralization; Nanomaterials; Nanotechnology; Reaction mechanisms; Research Article; Strategy; Thermal stability; thermal stability; artificial multienzyme; metal-organic frameworks; electrochemical assisted biomimetic mineralization; glucose detection
• ISSN: 1998-0124; 1998-0000
• DOI: 10.1007/s12274-019-2548-8

Recently, metal-organic framework (MOF)-based multienzyme systems integrating different functional natural enzymes and/or nanomaterial-based artificial enzymes are attracting increasing attention due to their high catalytic efficiency and promising application in sensing. Simple and controllable integration of enzymes or nanozymes within MOFs is crucial for achieving efficient cascade catalysis and high stability. Here, we report a facile electrochemical assisted biomimetic mineralization strategy to prepare an artificial multienzyme system for efficient electrochemical detection of biomolecules. By using the GO
x
@Cu-MOF/copper foam (GO
x
@Cu-MOF/CF) architecture as a proof of concept, efficient enzyme immobilization and cascade catalysis were achieved by
in situ
encapsulation of glucose oxidase (GO
x
) within MOFs layer grown on three-dimensional (3D) porous conducting CF via a facile one-step electrochemical assisted biomimetic mineralization strategy. Due to the bio-electrocatalytic cascade reaction mechanism, this well-designed GO
x
@Cu-MOF modified electrode exhibited superior catalytic activity and thermal stability for glucose sensing. Notably, the activity of GO
x
@Cu-MOF/CF still remained at ca. 80% after being incubated at 80 °C. In sharp contrast, the activity of the unprotected electrode was reduced to the original 10% after the same treatment. The design strategy presented here may be useful in fabricating highly stable enzyme@MOF composites applied for efficient photothermal therapy and other platform under high temperature.