Effect of calcium ions on the Hsp104 synthesis and heat tolerance of Saccharomyces cerevisiae

• Published in: Microbiology (New York) Vol. 79; no. 2; pp. 153 - 159
• Main Authors: Fedoseeva, I. V, Varakina, N. N, Rusaleva, T. M, Borovskii, G. B, Rikhvanov, E. G, Voinikov, V. K
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01-04-2010; SP MAIK Nauka/Interperiodica
• Subjects: Biomedical and Life Sciences; Experimental Articles; Life Sciences; Medical Microbiology; Microbiology; Saccharomyces cerevisiae; heat tolerance; calcium; Hsp104; mitochondria
• ISSN: 0026-2617; 1608-3237
• DOI: 10.1134/S0026261710020049

Effect of calcium ions on heat tolerance of Saccharomyces cerevisiae and on the induction of Hsp104 synthesis by this microorganism was studied. Short-term (30 min) treatment with CaCl₂ at 30°C enhanced the heat tolerance to the lethal heat shock (50°C); the synthesis of Hsp104 was induced as well. The effect of Ca²⁺ on the heat tolerance and Hsp104 synthesis was shown to be ion-specific and was inhibited by LaCl₃, which is known to block calcium ion channels on the cytoplasmic membrane. The effect of Ca²⁺ depended on the potential of the inner mitochondrial membrane. When the cells were treated with sodium azide, which reduced the electrochemical potential, the effect of calcium both on heat tolerance and Hsp104 synthesis was suppressed. Depending on the concentration of exogenous Ca²⁺ and the ambient conditions, calcium ions may either induce or inhibit the expression of the stress genes and cell viability.