Expression pattern analysis of key genes related to anther development in a mutant of male-sterile Betula platyphylla Suk

• Published in: Tree genetics & genomes Vol. 16; no. 2
• Main Authors: Xin, Qiqi, Hu, Xiaoqing, Zhang, Yong, Li, Dou, Xu, Bohan, Liu, Xuemei
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-04-2020; Springer Nature B.V
• Subjects: ABC transporters; Anthers; Betula platyphylla; Biomedical and Life Sciences; Biotechnology; Cytochrome; Cytochrome P450; Cytochromes P450; Cytoplasm; Developmental stages; Enzymes; Forestry; Gene expression; Genes; Genomes; Kinases; Life Sciences; Lipids; Male sterility; Males; Microspores; Molecular modelling; Morphology; Mutants; Original Article; Pattern analysis; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Pollen; Pollination; Proteins; Transcription; Transcriptomes; Tree Biology; Trees; Vacuoles; Male sterility; Birch; Microspore development; Gene expression pattern
• ISSN: 1614-2942; 1614-2950
• DOI: 10.1007/s11295-020-1425-7

Male-sterile mutants are ideal materials for studying the gene regulatory pathway of anther development. Here, the cytological morphology of mutant and wild-type anthers during the whole development stage was analyzed. At the microspore developmental stage, the tapetum cells of two types of mutants (mutant male inflorescences (MM), normal-like male inflorescences (NLM) showed additional vacuoles and a reduced cytoplasm, while the wild-type tapetum cells began to degenerate. Before pollination, compared with formed mature pollen grains in wild-type male inflorescences, collapsed microspores were discovered in mutants. Based on the transcriptome data of wild-type male inflorescence (NM) and mutant MM, ten key differentially expressed genes (
ABORTED MICROSPORES
(
AMS
),
MYB38
,
MALE STERILITY 1
(
MS1
),
MYB32
,
ODORANT1
(
ODO1
),
QUARTET3
(
QRT3
) and
CYTOCHROME P450 77A3
(
CYP77A3
),
ATP-Binding Cassette Transporter G26
(
ABCG26
),
FIL1
,
LIPID-TRANSFER PROTEIN 14 (LTP14)
) related to anther development were selected for further study. Furthermore,
MYB32
,
MYB38
,
AMS
,
MS1
, and
ABCG26
were highly expressed at the tetrad stage in MM and NLM, along with
ODO1
,
QRT3
, and
CYP77A3
, which were significantly upregulated at the tetrad stage in NLM,
FIL1
and
LTP14
were upregulated at the tetrad stage in MM. These events suggested that these genes may accelerate the abortion process in mutant birch anther. Tissue-specific expression analysis of ten genes indicated diverse spatiotemporal expression patterns.
QRT3
and
CYP77A3
were expressed in all five tissues, including leaves, stems, roots, female inflorescences, and male inflorescences, while the other eight genes had high transcription levels during male development, indicating that these genes may play different roles in these developmental processes. These results facilitate further investigation of the functional and molecular mechanisms underlying anther and microspore development in
Betula platyphylla.