CNT-Based Label-Free Electrochemical Sensing of Native DNA with Allele Single Nucleotide Polymorphism

• Published in: Semiconductors (Woodbury, N.Y.) Vol. 52; no. 14; pp. 1836 - 1838
• Main Authors: Grushevskaya, H. V., Krylova, N. G., Lipnevich, I. V., Babenka, A. S., Egorova, V. P., Chakukov, R. F.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-12-2018; Springer Nature B.V
• Subjects: ANIMAL TISSUES; Bundles; Carbon; CARBON NANOTUBES; Deoxyribonucleic acid; DIPOLES; DNA; ELECTROCHEMISTRY; ELECTRODES; ELECTRONS; FERMI LEVEL; Genetic testing; HIGH SPIN STATES; LAYERS; Magnetic Materials; Magnetism; METALS; NANOSCIENCE AND NANOTECHNOLOGY; Nanostructure Devices; NEOPLASMS; Nucleic acids; NUCLEOTIDES; ONCOGENES; OSCILLATIONS; Physics; Physics and Astronomy; PLASMONS; Polymorphism; PROBES; Selectivity; SENSITIVITY; SENSORS; SIGNALS; STABILIZATION; SURFACE COATING; SURFACES
• ISSN: 1063-7826; 1090-6479
• DOI: 10.1134/S1063782618140117

A label-free high-performance electrochemical sensing of single nucleotide polymorphism in native genomic DNA samples from cancer tissues, in oncogene-specific complementary DNA and in amplicons has been carried out on bundles of few-walled carboxylated carbon nanotubes, surface of which was coated with electron-dense layer of probe DNA. High selectivity and sensitivity of the novel DNA-nanosensor are ensured by the following features. Firstly, a complementary-hybridization signal is enhanced by resonance between plasmons in carbon nanotubes and dipole oscillations of hydrated nucleic acids located in near-electrode Helmholtz double layer of the impedance sensor. Secondly, a decoration of carbon nanotubes by adhered metal atoms in a high-spin state leads to Fermi level stabilization for the carbon nanotube bundles.