Measurement of infarct size and percentage myocardium infarcted in a dog preparation with single photon-emission computed tomography, thallium-201, and indium 111-monoclonal antimyosin Fab

• Published in: Circulation (New York, N.Y.) Vol. 76; no. 1; pp. 181 - 190
• Main Authors: JOHNSON, L. L, LERRICK, K. S, COROMILAS, J, SELDIN, D. W, ESSER, P. D, ZIMMERMAN, J. M, KELLER, A. M, ALDERSON, P. O, BIGGER, J. T. JR, CANNON, P. J
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD Lippincott Williams & Wilkins 01-07-1987
• Subjects: Animals; Antibodies, Monoclonal - immunology; Biological and medical sciences; Cardiology. Vascular system; Coronary heart disease; Dogs; Heart; Immunoglobulin Fab Fragments - immunology; Indium; Male; Medical sciences; Myocardial Infarction - diagnostic imaging; Myosins - immunology; Radioisotopes; Staining and Labeling; Tetrazolium Salts; Thallium; Tomography, Emission-Computed; Radionuclide study; Fissipedia; Carnivora; Infarct; Cardiovascular disease; Monoclonal antibody; Fab-Fragment; Coronary heart disease; Proteins; Vertebrata; Experimental disease; Mammalia; Myosin; Myocardium; Dog; Positron emission tomography
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/01.CIR.76.1.181

Single photon-emission tomography (SPECT) and indium 111-labeled monoclonal antimyosin Fab fragments were used to measure myocardial infarct size in 12 dogs, six subjected to balloon catheter-induced coronary artery occlusion for 6 hr (late reperfusion) and six subjected to occlusion with reperfusion at 2 hr (early reperfusion). Tomographic imaging was performed 24 hr after the intravenous injection of labeled Fab fragments with the use of a dual-head SPECT camera with medium-energy collimators. Immediately after the first tomographic scan, thallium-201 was injected into nine of 12 dogs and imaging was repeated. Estimated infarct size in grams was calculated from transaxially reconstructed, normalized, and background-corrected indium SPECT images with the use of a threshold technique for edge detection. Estimated noninfarcted myocardium in grams was calculated from obliquely reconstructed thallium SPECT images by a similar method. The animals were killed and infarct size in grams and true infarct size as a percentage of total left ventricular myocardial volume were measured by triphenyl tetrazolium chloride staining. Estimated infarct size from indium SPECT images showed an excellent correlation with true infarct size (r = .95, SEE = 4.1 g). Estimated percentage myocardium infarcted was calculated by dividing estimated infarct size from indium images by the sum of estimated infarct size plus estimated noninfarcted myocardium obtained from thallium images. Correlation between the estimated percentage of myocardium infarcted and true percentage of myocardium infarcted was excellent (r = .93, SEE = 4.4%). We conclude that dual-isotope SPECT with indium 111-monoclonal antimyosin antibodies and thallium-201 can accurately estimate infarct size and percentage myocardium infarcted.