Color Calibration Slide for Digital Microscopy Systems

• Published in: Measurement techniques Vol. 64; no. 10; pp. 852 - 856
• Main Authors: Makhov, D. S., Sagatelyan, G. R., Samorodov, A. V.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2022; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Calibration; Characterization and Evaluation of Materials; Chromaticity; Color; Comparative studies; Digital imaging; Health aspects; Measurement Science and Instrumentation; Microscope and microscopy; Microscopy; Physical Chemistry; Physics; Physics and Astronomy; Prototypes; Reproducibility; color calibration slide; calibration target; image standardization; whole slide imaging system; color correction; color calibration; digital microscopy
• ISSN: 0543-1972; 1573-8906
• DOI: 10.1007/s11018-022-02014-7

The article is dedicated to the image standardization problem in digital microscopy. Color reproducibility in microscopic images is of great importance as it can lead to therapeutic errors in image interpretation. Color reproducibility in digital microscopy systems with various hardware can be ensured with color calibration using a special slide. The proposed requirements for color slide development are based on the analysis of the literature. A color slide prototype consisting of 97 fields of colored glass has been developed and manufactured. Color chromaticity coordinates in CIE XYZ color space have been calculated from experimentally determined spectral transmitted radiation distributions. A comparative study of the proposed color slide prototype and existing color slide gamuts according to the most frequently used histological dyes chromaticity coordinates, has been carried out.