Investigation of the one-step electrochemical deposition of graphene oxide-doped poly(3,4-ethylenedioxythiophene)-polyphenol oxidase as a dopamine sensor

• Published in: RSC advances Vol. 12; no. 24; pp. 15575 - 15583
• Main Authors: Ramu, P, Vimal, S. P, Suresh, P, Sanmugam, Anandhavelu, Saravanakumar, U, Kumar, Raju Suresh, Almansour, Abdulrahman I, Arumugam, Natarajan, Vikraman, Dhanasekaran
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 23-05-2022; The Royal Society of Chemistry
• Subjects: Biosensors; Chemistry; Dopamine; Electrical measurement; Electrochemical analysis; Electrodes; Graphene; Oxidation; Polyphenol oxidase
• ISSN: 2046-2069; 2046-2069
• DOI: 10.1039/d2ra00791f

In this paper, we fabricated poly(3,4-ethylenedioxythiophene) (PEDOT)-graphene oxide-polyphenol oxidase (PEDOTGO-PPO) as a dopamine sensor. The morphology of PEDOT-GO-PPO was observed using scanning electron microscopy. Cyclic voltammetry was conducted to study the oxidation-reduction characteristics of dopamine. To optimize the pH, potential and limit of detection of dopamine, the amperometric technique was employed. The found limit of detection was 8 × 10
−9
M, and the linear range was from 5 × 10
−8
to 8.5 × 10
−5
M. The Michaelis-Menten constant (
K
m
) was calculated to be 70.34 μM, and the activation energy of the prepared electrode was 32.75 kJ mol
−1
. The electrode shows no significant change in the interference study. The modified electrode retains up to 80% of its original activity after 2 months. In the future, the biosensor can be used for the quantification of dopamine in human urine samples. The present modified electrode constitutes a tool for the electrochemical analysis of dopamine.
In this paper, we fabricated poly(3,4-ethylenedioxythiophene) (PEDOT)-graphene oxide-polyphenol oxidase (PEDOT-GO-PPO) as a dopamine sensor.