Aneuploidy in male Indian muntjac cells is limited to the Y2 chromosome

• Published in: Mutagenesis Vol. 13; no. 1; pp. 33 - 37
• Main Authors: Vig, Baldev K., Henderson, Amy
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-01-1998
• Subjects: Aneuploidy; Animals; Anthelmintics - pharmacology; benomyl; Benomyl - pharmacology; Biological and medical sciences; Caffeine - pharmacology; Cell Line; Centromere - genetics; Chemical mutagenesis; Colchicine - pharmacology; Gout Suppressants - pharmacology; Kinetochores - metabolism; Male; Medical sciences; Munticus muntiacus vaginalis; Muntjacs - genetics; Mutagens - pharmacology; Phosphodiesterase Inhibitors - pharmacology; Toxicology; X Chromosome - drug effects; X Chromosome - genetics; Y Chromosome - drug effects; Y Chromosome - genetics; Chromosomal aberration; Mutagenicity testing; Toxicity; Sex chromosome; Aneuploidy; Male; Benomyl; Muntiacus muntjak; Vertebrata; Mammalia; Cell line; Artiodactyla; Ungulata; Caffeine
• ISSN: 0267-8357; 1464-3804
• DOI: 10.1093/mutage/13.1.33

In untreated cell cultures of Indian muntjac (Munticus muntiacus vaginalis; 2n = 6♀, 7♂) we observed that spontaneous aneuploidy is limited primarily to the Y2 chromosome. We therefore treated the cells with aneugenic agents to determine if induced aneuploidy follows the same pattern and, hence, if there are limitations on the generation of aneuploidy or survival of cells lacking certain chromosomes. Exposing the cells to benomyl (8–100 μg/ml for 1 h), caffeine (5 × 10−5–2×10−4 for 2, 24 and 72 h) and colchicine (2 × 10−4 and 5 × 10−5 M for 1, 24, 48 and 72 h) resulted in cells primarily aneuploid for the Y2 chromosome. The frequency of cells lacking Y2 was far higher than those having an extra Y2 chromosome. The frequency of cells aneuploid for all other chromosomes combined was much lower than that for Y2. The data imply that the Indian muntjac genome can tolerate loss of the Y2 chromosome only and that aneuploidy for other chromosomes might cause lethality. This might be because the small number of chromosomes in the genome predisposes aneuploid cells to an imbalance of genes carrying out the basic activities required for cell division and cell survival. Because of the small chromosome number, the large size of the chromosomes and the ease of distinguishing every chromosome without banding or any other special treatment, e.g. FISH, this system could be useful and convenient in the study of induction of aneuploidy. This simple and inexpensive system can be utilized as a screening system for preliminary studies dealing with induced aneuploidy.