Changes in lung surfactant proteins in rats with lipopolysaccharide-induced fever

• Published in: Physiological research Vol. 63 Suppl 4; pp. S619 - S628
• Main Authors: Kolomaznik, M, Zila, I, Kopincova, J, Mokra, D, Calkovska, A
• Format: Journal Article
• Language: English
• Published: Czech Republic Institute of Physiology 01-01-2014
• Subjects: Aldosterone - blood; Animals; Body Temperature; Bronchoalveolar Lavage Fluid - chemistry; Experiments; Fever - metabolism; Fever - physiopathology; Laboratory animals; Leukocyte Count; Lipids; Lipopolysaccharides; Lungs; Male; Proteins; Pulmonary Surfactant-Associated Proteins - analysis; Pulmonary Surfactant-Associated Proteins - metabolism; Random Allocation; Rats, Wistar; Respiration; Rodents; Surfactants; Thiobarbituric Acid Reactive Substances - metabolism; Ventilation
• ISSN: 0862-8408; 1802-9973
• DOI: 10.33549/physiolres.932928

The study was designed to prove the hypothesis that lipopolysaccharide (LPS)-induced fever elicits the changes in surfactant specific proteins, potentially related to thermal tachypnea. In adult rats fever was induced by intraperitoneal administration of LPS at a dose 100 microg/kg of body weight; control group received saline. Respiratory parameters, arterial blood gases and pH and colonic body temperature (BT) were recorded. Five hours later, surfactant proteins (SP) A, B, C and D were evaluated in bronchoalveolar lavage fluid (BALF) and lung tissue (LT). LPS evoked monophasic thermic response (at 300 min 38.7+/-0.2 vs. 36.4+/-0.3 °C, P 0.05) and an increase in minute ventilation due to changes in breathing rate and tidal volume. LPS-instilled animals had higher levels of SP-A and SP-D in LT (P 0.05 and 0.01), and higher SP-D in BALF (P 0.01) than controls. SP-B increased in LT and SP-C in BALF of animals with LPS (both P 0.05 vs. controls). The changes in all surfactant specific proteins are present in LPS-induced fever. Alterations of proteins related to local immune mechanisms (SP-A, SP-D) are probably a part of general inflammatory response to pyrogen. Changes in proteins related to surface activity (SP-B and SP-C) might reflect the effort of the body to stabilize the lungs in thermal challenge.