Novel near-infrared fluorescence probe with large Stokes shift for monitoring CCl4-induced toxic hepatitis

• Published in: Talanta (Oxford) Vol. 223; p. 121720
• Main Authors: Tian, Yang, Zhou, Dong-Ye, Jiang, Wen-Li, She, Zun-Pan, Li, Yongfei, Li, Chun-Yan
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-02-2021
• Subjects: Fluorescent probe; Large Stokes shift; Near-infrared; Peroxynitrite; Toxic hepatitis; Large Stokes shift; Peroxynitrite; Toxic hepatitis; Fluorescent probe; Near-infrared
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2020.121720

Toxic hepatitis which is induced by chemical substance is a serious threat to human health. More and more studies have shown that peroxynitrite (ONOO−) is related with the development of toxic hepatitis. So it is important to find a tool to study ONOO− change during the diagnosis and therapy of toxic hepatitis. Herein, a series of novel near-infrared (NIR) fluorescence dyes (DDM-R) with long emission wavelength (740–770 nm) and large Stokes shift (~200 nm) are developed. Among the dyes, DDM-OH with great spectral performance and facilely modified feature is used to construct probe DDM-ONOO-. The probe have the preference of high sensitivity and excellent selectivity for ONOO−. In addition, DDM-ONOO- was applied in detecting exogenous and endogenous ONOO− in cells and further used in detecting ONOO− of CCl4-induced toxic hepatitis in cells by fluorescence imaging, 3D quantification analysis, flow cytometry. More importantly, by visualizing ONOO−, the probe was used to monitor the diagnosis of CCl4-induced toxic hepatitis in mice and evaluate the therapeutic efficacy of hepatoprotective medicines (NAC, SM, DDB). The results show that the probe will provide a powerful tool for the diagnosis and treatment of toxic hepatitis.
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•A series of novel fluorescence dyes which could be viewed as important biomaterials are developed.•This is the first time to study toxic hepatitis by fluorescence imaging.•The probe was applied in monitoring the diagnosis of toxic hepatitis in cells and mice.•The probe was used in evaluating the treatment of hepatoprotective medicines in cells and mice.