Chromosomal localization of a human cDNA containing a DIDS binding domain and demonstrating high homology to yeast omnipotent suppressor 45

• Published in: Somatic cell and molecular genetics Vol. 18; no. 1; pp. 97 - 102
• Main Authors: GRENETT, H. E, EIPERS, P. G, KIDD, V. J, BOUNELIS, P, FULLER, G. M
• Format: Journal Article
• Language: English
• Published: London Plenum 1992; New York, NY
• Subjects: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - analogs & derivatives; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid - metabolism; Amino Acid Sequence; Animals; Binding Sites; Biological and medical sciences; cDNA; Chickens; chromosome 5; chromosome 6; chromosome 7; Chromosome Mapping; Chromosomes, Human - chemistry; Chromosomes, Human, Pair 6 - chemistry; Chromosomes, Human, Pair 7 - chemistry; Classical genetics, quantitative genetics, hybrids; Cricetinae; Cricetulus; DNA - analysis; Fundamental and applied biological sciences. Psychology; genes; Genes, Suppressor; Genetics of eukaryotes. Biological and molecular evolution; Human; Humans; localization; man; Mice; Molecular Sequence Data; Saccharomyces cerevisiae - genetics; Sequence Homology, Nucleic Acid; Species Specificity; X chromosome; X Chromosome - chemistry; Genetic mapping; Adenocarcinoma; Human; B5-Chromosome; Tumor; C6-Chromosome; X-Chromosome
• ISSN: 0740-7750; 1572-9931
• DOI: 10.1007/BF01233452

We recently have identified a full-length cDNA (TB3-1) from a human adenocarcinoma cell line T84 cDNA library that encodes a 47.8-kDa protein. TB3-1 shares identity with the putative yeast translation termination factor omnipotent suppressor 45. Using human-mouse somatic cell panel analysis, a family of sequences with high homology to the TB3-1 cDNA clone were localized to human chromosomes 5, 6, 7, and X. Southern analysis of a panel of mammalian and chicken genomic DNA demonstrates that TB3-1 is well conserved in higher vertebrates.