Analysis of Bioactive Saponins in Fructus Gleditsiae abnormalis and Fructus Gleditsiae sinensis by LC-ELSD

• Published in: Chromatographia Vol. 70; no. 9-10; pp. 1361 - 1366
• Main Authors: Xia, Yufeng, Gao, Zhengzhen, Dai, Yue, Wang, Qiang
• Format: Journal Article
• Language: English
• Published: Wiesbaden Wiesbaden : Vieweg Verlag 01-11-2009; Vieweg Verlag; Springer
• Subjects: Analytical Chemistry; Biological and medical sciences; Chemistry; Chemistry and Materials Science; Chromatography; General pharmacology; Laboratory Medicine; Medical sciences; Original; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Pharmacy; Proteomics; Asia; China; Column liquid Chromatography; Saponins; Fructus; Evaporative light scattering detection; Quantitative analysis; Pentacyclic compound; Gleditsia sinensis; Chemical analysis; HPLC chromatography; Fructus Gleditsiae sinensis; Medicinal plant; Saponin; Folk medicine; Fructus Gleditsiae abnormalis; Dicotyledones; Angiospermae; Glycoside; Trisaccharide; Validation; Terpenoid; Fruit; Pharmacognosy; Oleanane derivatives; Leguminosae; Quality control; Simultaneous measurement; Plant origin; Triterpene; Active ingredient; Spermatophyta; Evaporative light scattering detector; Tetrasaccharide
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-009-1312-8

Fructus Gleditsiae abnormalis and Fructus Gleditsiae sinensis are widely used in traditional Chinese medicine. As different developmental stages of fruits of the same plant Gleditsia sinensis Lam., whether Fructus Gleditsiae sinensis can be used as the substitute of Fructus Gleditsiae abnormalis has long been debated. To compare the differences of the content of main active saponins between the two fruits, we established a new LC-ELSD method for simultaneous determination of four major saponins present in two fruits. After fully validated, the method was successfully applied to quantitatively analyze the four bioactive saponins of twenty samples, 11 of Fructus Gleditsiae abnormalis and 9 of Fructus Gleditsiae sinensis obtained from different locations. It was shown that there were no significant differences in saponins contents and LC profiles between the two fruits. Moreover, the developed method can be used for quality control of Fructus Gleditsiae abnormalis and Fructus Gleditsiae sinensis.