Efficient RecABC-dependent, homologous recombination between coliphage lambda and plasmids requires a phage ninR region gene

Efficient RecABC-dependent, homologous recombination between coliphage lambda and plasmids requires a phage ninR region gene

A phage lambda gene that gives a 100-fold increase in recombinant frequencies for RecABC pathway-mediated, phage-plasmid homologous recombination (Shen and Huang 1986) maps to ninG (orf 204) of lambda. We call this gene rap, for recombination adept with plasmid. A similar determinant exists in Charo...

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Bibliographic Details
Published in:Molecular & general genetics Vol. 210; no. 2; p. 248
Main Authors: Hollifield, W C, Kaplan, E N, Huang, H V
Format: Journal Article
Language:English
Published: Germany 01-12-1987
Subjects:
Bacteriophage lambda - genetics
Chromosome Mapping
Escherichia coli - genetics
Exodeoxyribonuclease V
Exodeoxyribonucleases - genetics
Genes, Viral
Plasmids
Rec A Recombinases - genetics
Recombination, Genetic
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Summary:A phage lambda gene that gives a 100-fold increase in recombinant frequencies for RecABC pathway-mediated, phage-plasmid homologous recombination (Shen and Huang 1986) maps to ninG (orf 204) of lambda. We call this gene rap, for recombination adept with plasmid. A similar determinant exists in Charon 4A and maps in phi 80-derived sequences, between nin5 and the Rz homology with lambda. The absence of the Rap+ phenotype from certain lambda vectors explains the inefficiency of screening the resulting phage libraries using phage-plasmid homologous recombination. The mapping of rap permits the construction of lambda vectors more suitable for this screening technique.
ISSN:0026-8925
DOI:10.1007/BF00325690