Glioma Proliferation Modulated in Vitro by Isothermal Radiofrequency Radiation Exposure

• Published in: Radiation research Vol. 121; no. 1; pp. 38 - 45
• Main Authors: Cleary, Stephen F., Liu, Li-Ming, Merchant, Randall E.
• Format: Journal Article
• Language: English
• Published: Oak Brook, Il Academic Press, Inc 01-01-1990; Radiation Research Society
• Subjects: ANIMAL CELLS; Biological and medical sciences; BIOLOGICAL EFFECTS; Biological effects of radiation; Cell cycle; Cell Division; Cell growth; Cell Line; Cell membranes; CELL PROLIFERATION; CHO cells; DISEASES; DNA REPLICATION; DNA, Neoplasm - biosynthesis; Dose response relationship; ELECTROMAGNETIC RADIATION; FREQUENCY RANGE; Fundamental and applied biological sciences. Psychology; Glioma; Glioma - pathology; GLIOMAS; Humans; IN VITRO; In Vitro Techniques; Lymphocytes; MHZ RANGE; NEOPLASMS; Non ionizing radiations. Hertzian waves. Biooptics; NUCLEIC ACID REPLICATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; P values; Radiation dosage; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RADIATIONS 560400 > Other Environmental Pollutant Effects; Radio Waves; RADIOWAVE RADIATION; RNA; RNA, Neoplasm - biosynthesis; Space life sciences; Statistical significance; Temperature; Tissues, organs and organisms biophysics; TUMOR CELLS; Tumor Cells, Cultured - radiation effects; Human; Cell culture; Radiobiology; Glioma; RNA synthesis; Radiofrequency irradiation; Isotherm; DNA synthesis; Tumor; Dose activity relation
• ISSN: 0033-7587; 1938-5404
• DOI: 10.2307/3577561

Isothermal (37 ± 0.2°C) exposure of glioma cells (LN71) for 2 h to 27 or 2450 MHz continuous-wave radiofrequency (RF) radiation in vitro modulated the rates of DNA and RNA synthesis 1, 3, and 5 days after exposure. The alterations indicate effects on cell proliferation and were not caused by RF-induced cell heating. The dose response for either frequency of the radiation was biphasic. Exposure to specific absorption rates (SARs) of 50 W/kg or less stimulated incorporation rates of tritiated thymidine (3 H- TdR) and tritiated uridine (^{3}{\rm H}\text{-}{\rm UdR}$), whereas higher SARs suppressed DNA and RNA synthesis. Statistically significant time-dependent alterations were detected for up to 5 days postexposure, suggesting a kinetic cellular response to RF radiation and the possibility of cumulative effects on cell proliferation. General mechanisms of effects are discussed.