Diagnostic value of the basophil activation test in evaluating Hymenoptera venom sensitization

• Published in: Wiener Klinische Wochenschrift Vol. 121; no. 9-10; pp. 344 - 348
• Main Authors: Peternelj, Andreja, Silar, Mira, Bajrovic, Nissera, Adamic, Katja, Music, Ema, Kosnik, Mitja, Korosec, Peter
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag 2009
• Subjects: Adolescent; Adult; Aged; Animals; Basophil Degranulation Test; Bee Venoms - immunology; Bites and Stings - immunology; Endocrinology; Epitopes - immunology; Female; Gastroenterology; Humans; Hymenoptera - immunology; Hypersensitivity - diagnosis; Hypersensitivity - immunology; Immunoglobulin E - blood; Internal Medicine; Intradermal Tests; Male; Mast Cells - immunology; Medicine; Medicine & Public Health; Middle Aged; Original Article; Pneumology/Respiratory System; Predictive Value of Tests; Prospective Studies; Sensitivity and Specificity; Wasp Venoms - immunology; Young Adult; CD63; Hymenoptera venom hypersensitivity; Basophil activation test
• ISSN: 0043-5325; 1613-7671
• DOI: 10.1007/s00508-009-1174-y

Summary
BACKGROUND: Diagnosis of allergy to Hymenoptera venom is usually confirmed with skin testing and measurement of specific serum IgE antibody, tests which are sometimes inconclusive. In these cases, additional in vitro tests are necessary. The aim of this study was to show the applicability of the basophil activation test in detecting sensitization to Hymenoptera venom and to compare the test sensitivity and clinical positive-predictive value with skin prick tests and measurement of allergen-specific serum IgE. METHODS: This prospective study was conducted between June 2004 and December 2007 and included a large group of 204 patients. All patients had a history of at least one systemic allergic reaction of Müller grades II–IV after a Hymenoptera sting. We compared results of the basophil activation test, specific serum IgE and skin prick tests with patients' clinical history and data on culprit insects. RESULTS: The overall clinical sensitivities of the basophil activation test, specific serum IgE and skin prick tests were 90%, 76% and 64%, respectively; the clinical positive-predictive values of the three tests were 79%, 73% and 78% for bee venom, 86%, 59% and 43% for wasp venom; and 84%, 77% and 22% for both venoms. CONCLUSIONS: Our results revealed a higher clinical sensitivity and comparable or better clinical positive-predictive value of basophil activation tests than skin prick tests and allergen-specific serum IgE in the detection of allergy to Hymenoptera venom.