Bradykinin-responsive cells of dorsal root ganglia in culture : cell size, firing, cytosolic calcium, and substance P

• Published in: Cellular and molecular neurobiology Vol. 14; no. 1; pp. 49 - 57
• Main Authors: KANO, M, KAWAKAMI, T, HIKAWA, N, HORI, H, TAKENAGA, T, GOTOH, H
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01-02-1994
• Subjects: Animals; Biological and medical sciences; Bradykinin - pharmacology; Calcium - metabolism; Cells, Cultured; Central nervous system; Cytosol - metabolism; Electrophysiology; Electrophysiology - methods; Fundamental and applied biological sciences. Psychology; Fura-2; Ganglia, Spinal - cytology; Ganglia, Spinal - physiology; Immunohistochemistry; Kinetics; Membrane Potentials - drug effects; Mice; Mice, Inbred C57BL; Neurons - cytology; Neurons - drug effects; Neurons - physiology; Spectrometry, Fluorescence; Substance P - analysis; Substance P - metabolism; Vertebrates: nervous system and sense organs; Cell culture; Spinal cord; Calcium; Substance P; Rodentia; Discharge pattern; Central nervous system; Electrophysiology; Dorsal ganglion; Vertebrata; Mammalia; Mouse; Bradykinin
• ISSN: 0272-4340; 1573-6830
• DOI: 10.1007/BF02088588

1. We analyze bradykinin-sensitive cells of the mouse dorsal root ganglion in culture from the viewpoints of cell size, electrical responses, and Ca2+ concentration change due to bradykinin and immunocytochemistry of substance P. 2. Sixteen percent of cells in the cell group 26-30 microns in diameter fired in response to 10 microM bradykinin. None of other cell groups showed a firing response to bradykinin. 3. We measured a cytosolic Ca2+ change due to bradykinin using a Ca(2+)-sensitive fluorescent dye, Fura 2. The rapid rise (peak time, 20 sec) in the Ca2+ concentration was ascribed to Ca2+ release from intracellular Ca2+ stores. The profound change in the Ca2+ concentration was observed again in the cell group 26-30 microns in diameter. Seventeen percent of cells in this group increased the Ca2+ concentration by approximately seven times that at resting level. 4. Among cells which increase Ca2+ concentration responding to bradykinin, 83% of them contain substance P (an immunocytochemical study). 5. We conclude that 16-17% of the cell group 26-30 microns in diameter of the dorsal root ganglia in culture are polymodal nociceptors and respond to bradykinin.