Antimicrobial metabolites produced by endophytic fungi associated with the leaves of Vochysia divergens

• Published in: Natural product research Vol. 38; no. 6; pp. 978 - 985
• Main Authors: Souza, Jéssica A. de M., Gubiani, Juliana R., de Siqueira, Kátia A., de Camargo, Maria J., Garcez, Walmir S., de Sousa, Paulo T., Soares, Marcos A., Araújo, Ângela R., Nunes, Emanuel V. dos S., Vieira, Lucas C. C., Sampaio, Olívia M., Goulart, Letícia S., Biasetto, Carolina R., de Menezes, Orivaldo T., de Oliveira, Camila M., Nogueira, Cláudio R., Pinto, Luciano da S., Teles, Helder L.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 18-03-2024; Taylor & Francis Ltd
• Subjects: antibacterial activity; Antibacterial agents; Antiinfectives and antibacterials; Antimicrobial activity; Divergence; Endophytes; Endophytic fungi; Fungi; Mass spectrometry; Mass spectroscopy; Metabolites; Microorganisms; Opportunist infection; Vochysia divergens; antibacterial activity; Endophytic fungi; Vochysia divergens
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2023.2208723

Investigation of the endophytic fungi Nigrospora sphaerica, Nigrospora oryzae, and Pseudofusicoccum stromaticum MeOH fractions isolated from the leaves of Vochysia divergens, a medicinal species from the Brazilian Pantanal, led to the identification of five compounds, namely a new compound (1E,8Z)-10,11-dihydroxy-5,5,8-trimethyl-4-oxocycloundeca-1,8-diene-1-carbaldehyde (1) and four known compounds: 5-methylmellein (2), sclerone (3), daldinone A (4), and lasiodiplodin (5). All compounds were identified using spectroscopic methods, and 1 was corroborated with mass spectrometry, while the known compounds were compared with data in the literature. The relative configuration of compound 1 was determined based on theoretical conformational studies as well as the J experimental values between the hydroxymethyne hydrogens. The antimicrobial activity of the compounds was evaluated. Promising results were obtained for compounds 2, 4, and 5 since they inhibited the bacterium Pseudomonas aeruginosa, an opportunistic pathogen, suggesting the potential of these microorganisms as a source of new antibacterial agents.