Exploratory lipidome and metabolome profiling contributes to understanding differences in high and normal ultimate pH beef

• Published in: Meat science Vol. 194; p. 108978
• Main Authors: Antonelo, Daniel S., dos Santos-Donado, Priscila R., Ferreira, Christina R., Colnago, Luiz A., Ocampos, Fernanda M.M., Ribeiro, Gabriel H., Ventura, Ricardo V., Gerrard, David E., Delgado, Eduardo F., Contreras-Castillo, Carmen J., Balieiro, Julio C.C.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-12-2022
• Subjects: Beef quality; Lipidomics; Metabolomics; Muscle metabolism; Nellore; Omics; Beef quality; Metabolomics; Lipidomics; Muscle metabolism; Omics; Nellore
• ISSN: 0309-1740; 1873-4138; 1873-4138
• DOI: 10.1016/j.meatsci.2022.108978

The aim of this work was to compare the lipidome and metabolome profiling in the Longissimus thoracis muscle early and late postmortem from high and normal ultimate pH (pHu) beef. Lipid profiling discriminated between high and normal pHu beef based on fatty acid metabolism and mitochondrial beta-oxidation of long chain saturated fatty acids at 30 min postmortem, and phospholipid biosynthesis at 44 h postmortem. Metabolite profiling also discriminated between high and normal pHu beef, mainly through glutathione, purine, arginine and proline, and glycine, serine and threonine metabolisms at 30 min postmortem, and glycolysis, TCA cycle, glutathione, tyrosine, and pyruvate metabolisms at 44 h postmortem. Lipid and metabolite profiles showed reduced glycolysis and increased use of alternative energy metabolic processes that were central to differentiating high and normal pHu beef. Phospholipid biosynthesis modification suggested high pHu beef experienced greater oxidative stress.
•Multiple reaction monitoring-profiling was used for lipid exploratory analysis.•Nuclear magnetic resonance spectroscopy was used to evaluate metabolic profile.•Lipid and metabolite profiles differentiate high and normal pHu beef.•High pHu beef has greater use of alternative energy metabolic processes.•High pHu beef has a greater response to cellular stress.