Qualitative and Quantitative NMR Approaches in Blood Serum Lipidomics

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1735; p. 365
• Main Authors: Barbosa, Banny Silva, Martins, Lucas Gelain, Costa, Tássia B B C, Cruz, Guilherme, Tasic, Ljubica
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Biomarkers; Humans; Lipids - blood; Magnetic Resonance Spectroscopy - methods; Metabolomics - methods; 1H NMR edited by diffusion; 1H NMR; Lipidomics; 1H qNMR; Blood serum lipids
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-7614-0_25

Nuclear magnetic resonance (NMR) spectroscopy in combination with chemometrics can be applied in the analysis of complex biological samples in many ways. For example, we can analyze lipids, elucidate their structures, determine their nutritional values, and determine their distribution in blood serum. As lipids are not soluble in water, they are transported in blood as lipid-rich self-assembled particles, divided into different density assemblies from high- to very-low-density lipoproteins (HDL to VLDL), or by combining with serum proteins, such as albumins (human serum albumins (HSA)). Therefore, serum lipids can be analyzed as they are using only a 1:1 (v/v) dilution with a buffer or deuterated water prior to analysis by applying
H NMR or
H NMR edited-by-diffusion techniques. Alternatively, lipids can be extracted from the serum using liquid partition equilibrium and then analyzed using liquid-state NMR techniques. Our chapter describes protocols that are used for extraction of blood serum lipids and their quantitative
H NMR (
H qNMR) analysis in lipid extracts as well as
H NMR edited by diffusion for direct blood serum lipid analysis.