New nystatin polymeric complexes and their in vitro antifungal evaluation in a model study with Fusarium oxysporum

• Published in: Mycopathologia (1975) Vol. 153; no. 1; pp. 15 - 19
• Main Authors: CHARVALOS, Ekatherina, TSATSAKIS, Aristeidis, TZATZARAKIS, Manolis, DOLAPSAKIS, George, STIAKAKIS, John
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 2002; Springer Nature B.V
• Subjects: Antibiotics. Antiinfectious agents. Antiparasitic agents; Antifungal agents; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Delayed-Action Preparations; Drug Carriers - chemistry; Drug Carriers - pharmacology; Drug dosages; Fundamental and applied biological sciences. Psychology; Fusarium - drug effects; Fusarium - growth & development; Fusarium - physiology; Hydrogen bonds; Medical sciences; Microbial Sensitivity Tests; Microbiology; Nystatin - chemistry; Nystatin - pharmacology; Pharmacology. Drug treatments; Povidone - chemistry; Povidone - pharmacology; Spores, Fungal - drug effects; Spores, Fungal - physiology; Statistical analysis; Toxicity; Greece; Athens Greece; Crete; Fusarium oxysporum; Growth; Germination; Model study; In vitro; Spores; Fungi; Water solubility; Models; Fungi Imperfecti; Effective dose; Inhibition; Molecular mass; Thallophyta; Nystatin
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1023/A:1015252121285

Six water-soluble nystatin-polyvilnylpyrrolidone complexes with respective MW of 10 kDa (NC1), 25 kDa (NC2), 30 kDa (NC3), 40 kda (NC4), 90 kDa (NC5), 360 kDa (NC6) were synthesized. The activity of the complexes was compared with that of nystatin against growth and spore germination of Fusarium oxysporum f.sp. radiciscucumerinum. The ED50 value (effective dose) of free nystatin in aqueous solution on growth inhibition on solid medium was determined at 35.7 ppm. The ED50 of the complexes NC3, NC4, NC5, and NC6 ranged from 2.2 to 4 times lower than that of nystatin. The NC6 complex exhibited the highest activity, followed by NC5, NC4, and NC3. The activities of NC1 and NC2 were about 3 and 1.7 times higher than nystatin respectively in the same in vitro model. The complexes NC6. NC1 and NC4 were 25.4, 13.6 and 6.9 times more active respectively than nystatin against spore germination of E oxysporum. The activity of the nystatin complexes was dependent on the molecular weight of the polymeric carrier.