Comparison of High-throughput and Manual DNA Extraction Methods for the Qualitative and Quantitative Analysis of MON810 Maize

• Published in: Food biotechnology Vol. 28; no. 3; pp. 232 - 249
• Main Authors: Grelewska-Nowotko, Katarzyna, Nowosielski, Jarosław, Żurawska-Zajfert, Magdalena, Czembor, Paweł Częstobor, Sowa, Sławomir
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis 01-01-2014; Taylor & Francis Ltd
• Subjects: Biotechnology; Comparative analysis; corn; corn flour; Deoxyribonucleic acid; DNA; DNA extraction; European Union; food biotechnology; Genetically altered foods; genetically modified organism (GMO); genetically modified organisms; GMO quantification; high-throughput DNA isolation; magnetic particles; Polymerase chain reaction; qualitative analysis; quantitative analysis; Zea mays; Europe
• ISSN: 1532-4249; 0890-5436; 1532-4249
• DOI: 10.1080/08905436.2014.931864

PCR-based methods are widely used in the European Union and in other countries for the detection, identification, and quantification of genetically modified organisms (GMOs). The preparation of good-quality DNA from plant samples for GMO detection can be a challenging task, particularly if the DNA will be used for quantitative analysis. Two DNA extraction methods, namely manual (NucleoSpin Food kit from Machery-Nagel) and high-throughput partially automated (NucleoMag Plant kit from Machery-Nagel) methods, which utilize different DNA separation principles, were used for the isolation of DNA from maize flour samples. Despite the higher DNA recovery obtained using the high-throughput isolation method, a lower PCR efficiency was achieved, most likely due to the presence of PCR inhibitors in the extracts. We found both DNA extraction methods suitable for GMO analysis.