Comparison of induction frequency, maturation capacity and germination of Abies numidica during secondary somatic embryogenesis

• Published in: Biologia plantarum Vol. 50; no. 4; pp. 785 - 788
• Main Authors: Vookova, B, Kormutak, A
• Format: Journal Article
• Language: English
• Published: 01-12-2006
• Subjects: 6-BENZILADENINA; 6-BENZYLADENINE; ABA; Abies; ABIES NUMIDICA; CULTIVO IN VITRO; CULTURE IN VITRO; EMBRIOGENESIS SOMATICA; EMBRION SOMATICO; EMBRYOGENESE SOMATIQUE; EMBRYON SOMATIQUE; EXPLANT; EXPLANTES; EXPLANTS; GERMINACION; GERMINATION; http://www.fao.org/aos/agrovoc#c_24135; http://www.fao.org/aos/agrovoc#c_24136; http://www.fao.org/aos/agrovoc#c_27520; http://www.fao.org/aos/agrovoc#c_27607; http://www.fao.org/aos/agrovoc#c_3; http://www.fao.org/aos/agrovoc#c_3247; http://www.fao.org/aos/agrovoc#c_33272; http://www.fao.org/aos/agrovoc#c_34114; http://www.fao.org/aos/agrovoc#c_36911; http://www.fao.org/aos/agrovoc#c_7495; http://www.fao.org/aos/agrovoc#c_754; IN VITRO CULTURE; IN VITRO REGENERATION; MICROPROPAGACION; MICROPROPAGATION; REGENERACION IN VITRO; REGENERATION IN VITRO; SACCHAROSE; SOMATIC EMBRYOGENESIS; SOMATIC EMBRYOS; SUCROSA; SUCROSE
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-006-0132-z

A method used to improve repetitive somatic embryogenesis and plant recovery of Algerian fir was evaluated by comparing induction frequency, maturation capacity and germination. Individual zygotic embryos differed only slightly in induction frequencies (6.8%) from somatic embryos of the first (5.7%) and second cycle (5.5-9.0%). The yield of mature embryos differed significantly among the cell lines of the same cycle as well as among the cell lines of different cycles. The percentage of abnormalities was the lowest in the first cycle of somatic embryos. The differences in germination of well developed somatic embryos depended on cell lines rather than on the cycle.